Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12760
ชื่อเรื่อง: Modulated expression of the HIV-1 2LTR zinc finger efficiently interferes with the HIV integration process
ผู้แต่ง: Moonmuang S.
Saoin S.
Chupradit K.
Sakkhachornphop S.
Israsena N.
Rungsiwiwut R.
Tayapiwatana C.
Keywords: doxycycline
lentivirus vector
protein 2LTR
tetracycline
unclassified drug
zinc finger protein
doxycycline
tetracycline
zinc finger protein
antiviral activity
Article
controlled study
gene expression
gene therapy
hematopoietic cell line
Human immunodeficiency virus 1 infection
nonhuman
pluripotent stem cell
protein expression
protein stability
SUP-T1 cell line
T lymphocyte
therapy effect
virus DNA cell DNA interaction
virus induction
virus interference
virus replication
dose response
drug effect
gene expression regulation
gene vector
genetics
HEK293 cell line
human
Human immunodeficiency virus 1
Human immunodeficiency virus infection
Lentivirus
long terminal repeat
pathogenicity
physiology
procedures
transgene
virology
virus DNA cell DNA interaction
Dose-Response Relationship, Drug
Doxycycline
Gene Expression Regulation
Genetic Therapy
Genetic Vectors
HEK293 Cells
HIV Infections
HIV Long Terminal Repeat
HIV-1
Humans
Lentivirus
Pluripotent Stem Cells
Tetracycline
Transgenes
Virus Integration
Zinc Fingers
วันที่เผยแพร่: 2018
บทคัดย่อ: Lentiviral vectors have emerged as the most efficient system to stably transfer and insert genes into cells. By adding a tetracycline (Tet)-inducible promoter, transgene expression delivered by a lentiviral vector can be expressed whenever needed and halted when necessary. Here we have constructed a doxycycline (Dox)-inducible lentiviral vector which efficiently introduces a designed zinc finger protein, 2-long terminal repeat zinc-finger protein (2LTRZFP), into hematopoietic cell lines and evaluated its expression in pluripotent stem cells. As a result this lentiviral inducible system can regulate 2LTRZFP expression in the SupT1 T-cell line and in pluripotent stem cells. Using this vector, no basal expression was detected in the T-cell line and its induction was achieved with low Dox concentrations. Remarkably, the intracellular regulatory expression of 2LTRZFP significantly inhibited HIV-1 integration and replication in HIV-inoculated SupT1 cells. This approach could provide a potential tool for gene therapy applications, which efficiently control and reduce the side effect of therapeutic genes expression. © 2018 The Author(s).
URI: https://ir.swu.ac.th/jspui/handle/123456789/12760
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85052957177&doi=10.1042%2fBSR20181109&partnerID=40&md5=bca53f2880a16d21efb8f71e053657a0
ISSN: 1448463
Appears in Collections:Scopus 1983-2021

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