Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12725
ชื่อเรื่อง: Plasmolipin, PmPLP1, from Penaeus monodon is a potential receptor for yellow head virus infection
ผู้แต่ง: Matjank W.
Ponprateep S.
Rimphanitchayakit V.
Tassanakajon A.
Somboonwiwat K.
Vatanavicharn T.
Keywords: protein plasmolipin
protein PLP1
proteolipid protein
unclassified drug
arthropod protein
myelin and lymphocyte protein
protein binding
Article
cell membrane
controlled study
disease predisposition
enzyme linked immunosorbent assay
immunoblotting
immunolocalization
mortality rate
nonhuman
Penaeus monodon
priority journal
protein expression
protein function
Sf9 cell line
virus neutralization
virus purification
Yellow head virus
animal
blood cell
cytology
gill
immunology
metabolism
Nidovirales infection
Penaeidae
Roniviridae
Spodoptera
upregulation
veterinary medicine
virology
Animals
Arthropod Proteins
Cell Membrane
Gills
Hemocytes
Myelin and Lymphocyte-Associated Proteolipid Proteins
Nidovirales Infections
Penaeidae
Protein Binding
Roniviridae
Sf9 Cells
Spodoptera
Up-Regulation
วันที่เผยแพร่: 2018
บทคัดย่อ: Plasmolipin has been characterized as a cell entry receptor for mouse endogenous retrovirus. In black tiger shrimp, two isoforms of plasmolipin genes, PmPLP1 and PmPLP2, have been identified from the Penaeus monodon EST database. The PmPLP1 is highly up-regulated in yellow head virus (YHV)-infected shrimp. Herein, the function of PmPLP1 is shown to be involved in YHV infection. The immunoblotting and immunolocalization showed that the PmPLP1 protein was highly expressed and located at the plasma membrane of gills from YHV-infected shrimp. Moreover, the PmPLP1 expressed in the Sf9 insect cells resided at the cell membrane rendering the cells more susceptible to YHV infection. Using the ELISA binding and mortality assays, the synthetic external loop of PmPLP1 was shown to bind the purified YHV and neutralize the virus resulting in the decrease in YHV infection. Our results suggested that the PmPLP1 was likely a receptor of YHV in shrimp. © 2018 Elsevier Ltd
URI: https://ir.swu.ac.th/jspui/handle/123456789/12725
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053082050&doi=10.1016%2fj.dci.2018.07.021&partnerID=40&md5=d8dfaab4037163415ab32e7d3d2fd25a
ISSN: 0145305X
Appears in Collections:Scopus 1983-2021

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