Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12706
ชื่อเรื่อง: Gas chromatography–mass spectrometry analysis, in vitro activities, and in silico molecular docking of major components of michelia alba dc essential oil and scented extracts
ผู้แต่ง: Khammee T.
Jaratrungtawee A.
Kuno M.
วันที่เผยแพร่: 2018
บทคัดย่อ: Objective: The essential oil and scented extracts of Michelia alba DC. were analyzed by gas chromatography–mass spectrometry (GC–MS) and investigated for antidiabetic activities in vitro and in silico. Methods: The identification of steam distilled essential oil and scented extracts of M. alba was performed by GC–MS on the Agilent 7890A chromatograph couple with GC-7000 Mass Triple Quadrupole. The extractions have been evaluated the antidiabetic activities by alpha-amylase (α-amylase) assay using starch as substrates. In addition, computational molecular docking analysis of significant components was studied to understanding how selected compounds interacted with α-amylase using AutoDock 4.2. Results: The yields of M. alba of steam distilled essential oil and solvent extractions including hexane, diethyl ether, and dichloromethane were 0.16%, 0.02%, 0.47%, and 0.92%, respectively. GC–MS analysis of essential oil revealed that the main component was monoterpenoids β-linalool (65.03%). Meanwhile, 2-methylbutanoic acid was a primary in hexane extract (36.54%) and dichloromethane extract (33.07%). In the case of ether extract, the primary compound was β-linalool (37.32%) same as in essential oil. The antidiabetic activities evaluation demonstrates that essential oil and scented extracts have shown promising α-amylase inhibition activity. Essential oil from steam distillation revealed the best inhibition potential with a half maximal inhibitory concentration value of 0.67±4.7 mg/ml and their significant components demonstrated negative binding energies, indicating a high affinity to the α-amylase-binding site using molecular docking simulation. Conclusion: Data from this study suggest that essential oil and scented extracts of M. alba DC possess in vitro α-amylase activities and can be used for therapy of diabetes. © 2018, Innovare Academics Sciences Pvt. Ltd. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12706
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058303326&doi=10.22159%2fajpcr.2018.v11i12.29043&partnerID=40&md5=40e0098c4a7d04869f8d3832e334068d
ISSN: 9742441
Appears in Collections:Scopus 1983-2021

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