Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12629
ชื่อเรื่อง: Stability, Cytotoxicity, and Retinal Pigment Epithelial Cell Binding of Hyaluronic Acid-Coated PLGA Nanoparticles Encapsulating Lutein
ผู้แต่ง: Chittasupho C.
Posritong P.
Ariyawong P.
Keywords: hyaluronic acid
nanoparticle
polyglactin
xanthophyll
hyaluronic acid
nanoparticle
xanthophyll
ARPE-19 cell line
Article
cell viability
controlled study
dispersity
drug binding
drug cytotoxicity
drug degradation
drug release
drug solubility
drug stability
in vitro study
nanoencapsulation
particle size
photodegradation
priority journal
retina pigment cell
retinal pigment epithelium
storage temperature
zeta potential
cell culture
cell survival
chemistry
drug effect
drug stability
epithelium cell
human
metabolism
retinal pigment epithelium
Cell Survival
Cells, Cultured
Drug Stability
Epithelial Cells
Humans
Hyaluronic Acid
Lutein
Nanoparticles
Polylactic Acid-Polyglycolic Acid Copolymer
Retinal Pigment Epithelium
วันที่เผยแพร่: 2019
บทคัดย่อ: The application of lutein was limited due to water insolubility and susceptible to heat and light degradation. In this study, hyaluronic acid (HA)-coated PLGA nanoparticles encapsulating lutein were fabricated by a solvent displacement method to improve the physicochemical properties and the stability of lutein. A biphasic release profile of lutein was observed, following zero-order release kinetics. The physical stability of lutein stored at 4°C, 30°C, and 40°C for 30 days was enhanced when lutein was encapsulated in the nanoparticles. The degradation of lutein in PLGA NPs coated with HA was fitted to a second-order kinetic model. The rate constant increased with increasing storage temperature. The activation energy of lutein-NPs was 63.26 kJ/mol. The half-lives of lutein in PLGA-NPs were about 49, 4, and 2 days at a storage temperature of 4°C, 30°C, and 40°C, respectively. The results suggested that lutein-NPs should be stored at 4°C to prevent physical and chemical degradation. The photodegradation of lutein in NPs followed a second-order kinetic model. The rate constant was 0.0155 mg -1  ml day -1 . Cell viability study revealed that HA-coated PLGA nanoparticles encapsulating lutein did not show toxicity against retinal pigment epithelial cells (ARPE-19). The NPs bound ARPE-19 cells in a time- and a dose-dependent manner. The binding efficiency of lutein-NPs decreased at higher concentrations, suggesting that the NPs might reach binding saturation capacity. In conclusion, HA-coated PLGA nanoparticles could be used to deliver lutein and improved physicochemical property of lutein. [Figure not available: see fulltext.]. © 2018, American Association of Pharmaceutical Scientists.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12629
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058760535&doi=10.1208%2fs12249-018-1256-0&partnerID=40&md5=e481f80c1bb8c9b84c97ff65c9cec643
ISSN: 15309932
Appears in Collections:Scopus 1983-2021

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