Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12408
ชื่อเรื่อง: Characterization of flagellotropic, chi-like salmonella phages isolated from thai poultry farms
ผู้แต่ง: Phothaworn P.
Dunne M.
Supokaivanich R.
Ong C.
Lim J.
Taharnklaew R.
Vesaratchavest M.
Khumthong R.
Pringsulaka O.
Ajawatanawong P.
Klumpp J.
Brown N.
Imam M.
Clokie M.R.J.
Galyov E.E.
Korbsrisate S.
Keywords: bacterium lipopolysaccharide
capsid protein
agricultural land
Article
bacterial genome
bacterium culture
DNA base composition
Escherichia coli
flagellum
gene amplification
gene sequence
genetic analysis
host range
Listeria monocytogenes
next generation sequencing
nonhuman
nucleotide sequence
phage titer
phylogenomics
poultry
Salmonella enterica serovar Agona
Salmonella enterica serovar Albany
Salmonella enterica serovar Altona
Salmonella enterica serovar Choleraesuis
Salmonella enterica serovar Corvalis
Salmonella enterica serovar Enteritidis
Salmonella enterica serovar Hadar
Salmonella enterica serovar Kentucky
Salmonella enterica serovar Saintpaul
Salmonella enterica serovar Schwarzengrund
Salmonella enterica serovar Singapore
Salmonella enterica serovar Typhimurium
Salmonella enterica serovar Virchow
Salmonella phage
Staphylococcus aureus
Thai (citizen)
Thailand
transmission electron microscopy
Vibrio
animal
classification
genetics
isolation and purification
microbiology
phylogeny
phylogeography
Siphoviridae
virology
virus genome
Animals
Farms
Genome, Viral
Phylogeny
Phylogeography
Poultry
Salmonella Phages
Salmonella typhimurium
Siphoviridae
Thailand
วันที่เผยแพร่: 2019
บทคัดย่อ: Despite a wealth of knowledge on Salmonella phages worldwide, little is known about poultry-associated Salmonella phages from Thailand. Here, we isolated 108 phages from Thai poultry farms that infect Salmonella enterica serovar Typhimurium. Phages STm101 and STm118 were identified as temperate Siphoviridae phages. Genome sequencing and analyses revealed these phages share approximately 96% nucleotide sequence similarity to phage SPN19, a member of the Chi-like virus genus. PCR amplification of the gene encoding capsid protein E of the Chi-like phage was positive for 50% of phage isolates, suggesting a predominance of this phage type among the sampled poultry farms. In addition to the flagella, two phages required the lipopolysaccharide to infect and lyse Salmonella. Furthermore, phylogenomic analysis demonstrated that phages STm101 and STm118 formed a monophyletic clade with phages isolated from Western countries, but not from closer isolated phages from Korea. However, further investigation and more phage isolates are required to investigate possible causes for this geographic distribution. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12408
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068166916&doi=10.3390%2fv11060520&partnerID=40&md5=48344426ae1772dddf2cacdb0f6d21dd
ISSN: 19994915
Appears in Collections:Scopus 1983-2021

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