Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12350
ชื่อเรื่อง: The inhibitory effect of 13-butoxyberberine bromide on migration in breast cancer MDA-MB-231 cells
ผู้แต่ง: Laomethakorn P.
Jaitrong M.
Samosorn S.
Watanapokasin R.
Keywords: 13 butoxyberberine bromide
berberine derivative
beta actin
chemoattractant
gelatinase A
gelatinase B
proteinase inhibitor
unclassified drug
Article
breast cancer
cell migration
cell migration assay
cell survival
cell viability
chemoluminescence
controlled study
down regulation
human
human cell
MDA-MB-231 cell line
metastasis
microscopy
migration inhibition
MTT assay
protein expression level
transwell chamber assay
Western blotting
wound healing assay
วันที่เผยแพร่: 2019
บทคัดย่อ: Background: 13-butoxyberberine bromide is a berberine derivative that has not been reported on the anti-migration mechanism of breast cancer. Cancer metastasis is the major cause of cancer mortality, about 90% of cancer patients death are caused by cancer metastasis not the primary tumor. Therefore, the compound that could inhibit cancer migration is necessary for development as anti-cancer drug. Objective: To investigate the effects of 13-butoxyberberine bromide on the anti-migration of breast cancer MDA-MB-231 cells. Materials and Methods: Cell viability was determined by MTT assay. Wound-healing assay and transwell chamber assay were used to determine cell migration. Protein expression levels were examined by western blot analysis. Results: 13-Butoxyberberine bromide at sub-toxic concentration (10 μg/mL) inhibited cell migration in MDA-MB-231 treated cells via down-regulation of MMP-2 and MMP-9 expression which play important role in cancer cell metastasis. Conclusion: 13-Butoxyberberine bromide at 10 μg/mL showed anti-migration effect against MDA-MB-231 cells through inhibition of mediator proteins. These findings could be useful in the development of 13-butoxyberberine bromide to be a novel anti-migration agent for clinical use in the future. © Journal Of The Medical Association Of Thailand| 2019.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12350
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070210485&partnerID=40&md5=c2f323af647ff770e880c7729323edf1
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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