Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11899
ชื่อเรื่อง: Ultraviolet B irradiation-induced keratinocyte senescence and impaired development of 3D epidermal reconstruct
ผู้แต่ง: Wattanapitayakula S.K.
Chularojmontri L.
Schäfer-Korting M.
Keywords: Epidermis
Keratinocytes
Reactive oxygen species
Senescence
Skin aging
Ultraviolet radiation
beta galactosidase
cyclin D2
cyclin dependent kinase inhibitor 2A
hydrogen peroxide
hydroxyl radical
interleukin 1beta
interleukin 6
mammalian target of rapamycin
peroxynitrite
reactive oxygen metabolite
superoxide
aging
Article
BrdU assay
cell division
cell isolation
cell proliferation
cell proliferation assay
cell survival
cell viability
controlled study
cutaneous parameters
DNA damage
DNA microarray
DNA repair
epidermis
flow cytometry
genomic instability
histology
human
human cell
keratinocyte
microscopy
oxidative stress
phenotype
photoaging
protein expression
radiation exposure
reverse transcription polymerase chain reaction
senescence
ultraviolet B radiation
วันที่เผยแพร่: 2021
บทคัดย่อ: Ultraviolet B (UVB) induces morphological and functional changes of the skin. This study investigated the effect of UVB on keratinocyte senescence and the development of reconstructed human epidermis (RHE). Primary normal human keratinocytes (NHK) from juvenile foreskin were irradiated with UVB (30 mJ cm–2) and these effects were compared to NHK that underwent senescence in the late passage. UVB enhanced the accumulation of reactive oxygen species (ROS) and halted cell replication as detected by BrdU cell proliferation assay. The senescence phenotype was evaluated by beta-galactosidase (β-gal) staining and qPCR of genes related to senescent regulation, i.e. p16INK4a, cyclin D2, and IFI27. Senescence induced by high dose UVB resulted in morphological changes, enhanced β-gal activity, elevated cellular ROS levels and reduced DNA synthesis. qPCR revealed differential expression of the genes regulated senescence. p16INK4a expression was significantly increased in NHK exposed to UVB whereas enhanced IFI27 expression was observed only in cultural senescence. The levels of cyclin D2 expression were not significantly altered either by UVB or long culturing conditions. UVB significantly induced the aging phenotype in keratinocytes and impaired epidermal development. RHE generated from UVB-irradiated keratinocytes showed a thinner cross-sectional structure and the majority of keratinocytes in the lower epidermis were degenerated. The 3D epidermis model is useful in studying the skin aging process. © 2021 Sciendo. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/11899
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095862250&doi=10.2478%2facph-2021-0011&partnerID=40&md5=1b90e9a62361109edb411890418d4e23
ISSN: 13300075
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.