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dc.contributor.authorNantaphol S.
dc.contributor.authorMoonla C.
dc.contributor.authorPromvichai S.
dc.contributor.authorTangkuaram T.
dc.contributor.authorChailapakul O.
dc.contributor.authorSiangproh W.
dc.description.abstractUltra-high-performance liquid chromatography (UHPLC) coupled with a cobalt phthalocyanine screen-printed carbon electrode (CoPc-SPCE) was developed and validated for quantitative analysis of ethylenethiourea (ETU) and propylenethiourea (PTU). CoPC-SPCE provided high catalytic properties for ETU and PTU oxidation. This fabricated electrode is inexpensive, disposable, and easy to prepare by an in-house screen-printing technique. The chromatographic separation was performed in isocratic mode on a reversed phase C18 (100 mm × 4.6 mm, 3 µm) column, using a 90?:?10 (v/v) ratio of 0.05 M phosphate buffer solution (pH 4) and methanol as the mobile phase with a flow rate of 1.0 mL min-1 at an oxidation potential of +0.7 V vs. Ag/AgCl. The separation could be achieved within 3 min, and a wide linear range of 0.01-100 µg mL-1 (r2 > 0.99) was obtained for both analytes. The limits of detection (3 S/N) were found to be 0.006 and 0.009 µg mL-1 for ETU and PTU, respectively. Furthermore, this proposed method was utilized to determine ETU and PTU in fruit samples with satisfactory results, yielding excellent intra-day and inter-day relative standard deviations and recoveries. These results demonstrated that the proposed assay can be used as a new alternative way for inexpensive, rapid, selective and sensitive determination of ETU and PTU in fruit samples. © The Royal Society of Chemistry.
dc.subjectCatalytic oxidation
dc.subjectHigh performance liquid chromatography
dc.subjectHigh pressure liquid chromatography
dc.subjectScreen printing
dc.subjectChromatographic separations
dc.subjectCobalt phthalocyanine
dc.subjectPhosphate buffer solutions
dc.subjectPropylenethiourea (PTU)
dc.subjectRelative standard deviations
dc.subjectScreen printing technique
dc.subjectScreen-printed carbon electrodes
dc.subjectUltra high performance liquid chromatography (UHPLC)
dc.titleA new alternative assay for sensitive analysis of ethylenethiourea and propylenethiourea in fruit samples after their separation
dc.identifier.bibliograpycitationAnalytical Methods. Vol 12, No.29 (2020), p.3705-3712
Appears in Collections:Scopus 1983-2021

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