Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11827
ชื่อเรื่อง: Modified Riceberry rice extract suppresses melanogenesis-associated cell differentiation through tyrosinase-mediated MITF downregulation on B16 cells and in vivo zebrafish embryos
ผู้แต่ง: Rodboon T.
Sirilun S.
Okada S.
Kariya R.
Chontananarth T.
Suwannalert P.
Keywords: dopachrome tautomerase
melanin
microphthalmia associated transcription factor
modified Riceberry rice extract
monophenol monooxygenase
oxygenase inhibitor
phenylthiourea
plant extract
tyrosinase related protein 1
unclassified drug
animal cell
animal experiment
animal model
animal tissue
antimelanogenic activity
Article
B16 cell line
cell differentiation
cell structure
controlled study
cytokinesis
cytotoxicity
down regulation
drug activity
embryo
enzyme activity
enzyme inhibition
female
fermentation
genotoxicity
germination
hyperpigmentation
in vivo study
male
melanogenesis
micronucleus test
mRNA expression level
mutagenic activity
mutagenicity
nonhuman
protein expression
protein expression level
rice
Riceberry rice
Saccharomyces cerevisiae
zebra fish
วันที่เผยแพร่: 2020
บทคัดย่อ: Excessive melanin production caused by overactive tyrosinase (TYR) enzyme results in several dermatological problems. The TYR inhibitor, derived from metabolite changes during fermentation, has been well recognized for pigmentation control. Experimental approach: This study is interested in alternative anti-melanogenic agents from bio-modified Riceberry rice through fermentation. Modified Riceberry rice extract (MRB) was evaluated for its cytotoxicity, melanin content, melanin excretion, and TYR activity in B16 cells. TYR and their melanogenesis-related molecules such as TYR-related proteins-1 and -2, and microphthalmia-associated transcription factor (MITF) were determined. The anti-melanogenic activity and toxicity were also tested using the embryonic zebrafish model. Furthermore, comprehensive genotoxicity testing was verified by cytokinesis-block micronucleus cytome assay. Findings/Results: The study found that non-cytotoxic concentrations of MRB at 20 and 40 mg/mL inhibited melanogenesis and melanin excretion by interfering B16 cell morphology. Cellular TYR enzymatic activity was also suppressed in the treated cells. The mRNA transcription and protein expression levels of TYR and MITF decreased by dose-dependent and time-dependent manners with MRB treatment. In the animal model, MRB was found to be safe and potent for melanogenesis-related TYR inhibition in embryonic zebrafish at 20 and 30 mg/mL. The toxicity of effective doses of MRB showed no genotoxicity and mutagenicity. Conclusion and implications: This study suggests that MRB has anti-melanogenesis potential through TYR and its-related protein inhibitions. MRB is also safe for applications and maybe a promising anti-melanogenic agent for hyperpigmentation control. © 2020 Wolters Kluwer Medknow Publications. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/11827
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85095132431&doi=10.4103%2f1735-5362.297852&partnerID=40&md5=d820f1f1c02cb26be6b8470e1ea1cb93
ISSN: 17355362
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.