Publication: Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor
2
0
Issued Date
2011
Resource Type
File Type
application/pdf
Other identifier(s)
2-s2.0-84860449445
Rights Holder(s)
Scopus
Bibliographic Citation
BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), p.86-88
Suggested Citation
Kaewphinit T., Santiwatanakul S., Jaratsing P., Chansiri K., Arunrut N., Kiatpathomchai W. Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick biosensor. BMEiCON-2011 - 4th Biomedical Engineering International Conference. (2011), p.86-88. doi:10.1109/BMEiCon.2012.6172024 Retrieved from: https://hdl.handle.net/20.500.14740/7189
Abstract
Tuberculosis is a persistent problem in the developing world and the biggest cause of mortality. Loop-mediated isothermal amplification (LAMP) allows DNA to be amplified rapidly at a constant temperature. Here a LAMP method was combined with a chromatographic lateral-flow dipstick (LFD) to detect IS6110 gene of M. tuberculosis rapidly and specifically. The reaction was optimized at 65°C for 90 min and amplified DNA hybridized to an FITC-labeled oligonucleotide probe for 5 min was detected at LFD test line 5 min after application. Excluding for the step of DNA extraction, test results could be generated within 1 h 40 min. In addition to advantages of short assay time, confirmation of amplicon identity by hybridization and elimination of electrophoresis with carcinogenic ethidium bromide, the LAMP-LFD was more sensitive than an existing PCR assay for detection of M. tuberculosis. © 2011 IEEE.
Subject(s)
Amplicons
Constant temperature
Developing world
DNA extraction
Ethidium bromide
Lateral Flow
Lateral flows
LFD
M. tuberculosis
Mycobacterium tuberculosis
Oligonucleotide probes
PCR assay
Test-lines
Biomedical engineering
Biosensors
Bromine compounds
Developing countries
Electric lamps
Electrophoresis
Genes
Isotherms
Oligonucleotides
Polymerase chain reaction
Tubes (components)
Constant temperature
Developing world
DNA extraction
Ethidium bromide
Lateral Flow
Lateral flows
LFD
M. tuberculosis
Mycobacterium tuberculosis
Oligonucleotide probes
PCR assay
Test-lines
Biomedical engineering
Biosensors
Bromine compounds
Developing countries
Electric lamps
Electrophoresis
Genes
Isotherms
Oligonucleotides
Polymerase chain reaction
Tubes (components)