Publication:
Inhibitory effects on chondrosarcoma cell metastasis by Senna alata extract

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

7533322

DOI

10.1016/j.biopha.2021.111337

Other identifier(s)

2-s2.0-85100802120

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Biomedicine and Pharmacotherapy. Vol 137, No. (2021)

Suggested Citation

Kittiwattanokhun A., Samosorn S., Innajak S., Watanapokasin R. Inhibitory effects on chondrosarcoma cell metastasis by Senna alata extract. Biomedicine and Pharmacotherapy. Vol 137, No. (2021). doi:10.1016/j.biopha.2021.111337 Retrieved from: https://hdl.handle.net/20.500.14740/7817

Title

Inhibitory effects on chondrosarcoma cell metastasis by Senna alata extract

Author(s)

Kittiwattanokhun A.
 Samosorn S.
 Innajak S.
 Watanapokasin R.

Abstract

Background: Senna alata L. Roxb or candle bush is a traditional medicinal plant with a wide range of biological activities including anti-inflammatory, antimicrobial and antifungal. Leaf extract of S. alata showed the anti-tumor activity in various cancer cell lines. In this study, we focused on the inhibitory mechanism of S. alata extract (SAE) on cancer metastasis including cell migration, cell invasion and signaling pathways in chondrosarcoma SW1353 cells. Purpose: This study aimed to evaluate the anti-metastatic mechanisms of Senna alata extract on chondrosarcoma SW1353 cells. Methods: Screening for phytochemicals in biologically active fraction of SAE was analysed by 1H NMR spectroscopy. Cell viability and cytoxicity were determined by using MTT assay. Cell migration was observed by scratch wound healing and transwell migration assay. Cell invasion and cell adhesion assay were examined by Matrigel coated transwell chambers or plates. The expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), MAPKs and PI3K/Akt signaling pathways and NF-κB were detected by Western blot analysis. Results: The SAE treatment at the sub-cytoxic and non-cytotoxic concentrations significantly inhibited cell migration, cell invasion and cell adhesion of SW1353 cells in a dose-dependent manner. The results from Western blot analysis showed decreased MMP-2 and MMP-9 expression, while increased TIMP-1 and TIMP-2 expression in SAE treated cells. Moreover, SAE suppressed phosphorylation of ERK1/2, p38 and Akt but decreased NF-κB transcription factor expression in SW1353 cells. Conclusion: These results revealed that SAE could reduce MMP-2 and MMP-9 expression by downregulation of NF-κB which is downstream of MAPKs and PI3K/Akt signaling pathway in SW1353 cells resulting in reduced cancer cell migration and invasion. Therefore, SAE may have the potential use as an alternative treatment of chondrosarcoma metastasis. © 2021

Subject(s)

Antineoplastic agent
 Chloroform
 Gelatinase A
 Gelatinase B
 Immunoglobulin enhancer binding protein
 Mitogen activated protein kinase 1
 Mitogen activated protein kinase 3
 Plant extract
 Senna alata extract
 Tissue inhibitor of metalloproteinase 1
 Tissue inhibitor of metalloproteinase 2
 Unclassified drug
 Gelatinase A
 Gelatinase B
 Immunoglobulin enhancer binding protein
 Mitogen activated protein kinase
 MMP2 protein, human
 MMP9 protein, human
 Oncoprotein
 Phosphatidylinositol 3 kinase
 Senna extract
 TIMP1 protein, human
 TIMP2 protein, human
 Tissue inhibitor of metalloproteinase 1
 Tissue inhibitor of metalloproteinase 2
 Article
 Cell adhesion assay
 Cell invasion
 Cell migration
 Cell proliferation
 Cell viability
 Chondrosarcoma
 Controlled study
 Down regulation
 Drug mechanism
 Drug screening
 Human
 Human cell
 In vitro study
 Metastasis
 Metastasis inhibition
 MTT assay
 Pi3K/Akt signaling
 Plant leaf
 Priority journal
 Protein expression
 Proton nuclear magnetic resonance
 SW1353 cell line
 Thin layer chromatography
 Transwell assay
 Tumor microenvironment
 Western blotting
 Wound closure
 Wound healing assay
 Cell adhesion
 Cell motion
 Cell survival
 Chemistry
 Chondrosarcoma
 Drug effect
 Metabolism
 Metastasis
 Signal transduction
 Tumor cell line
 Cell Adhesion
 Cell Line, Tumor
 Cell Movement
 Cell Proliferation
 Cell Survival
 Chondrosarcoma
 Humans
 Matrix Metalloproteinase 2
 Matrix Metalloproteinase 9
 Mitogen-Activated Protein Kinases
 Neoplasm Metastasis
 NF-kappa B
 Oncogene Protein v-akt
 Phosphatidylinositol 3-Kinase
 Senna Extract
 Signal Transduction
 Tissue Inhibitor of Metalloproteinase-1
 Tissue Inhibitor of Metalloproteinase-2

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URI

https://hdl.handle.net/20.500.14740/7817

Collections

Scopus: Year 1983-2021

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