Publication:
Anticancer and apoptosis-inducing activities of microbial metabolites

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

7173458

DOI

10.2225/vol13-issue5-fulltext-7

Other identifier(s)

2-s2.0-79960762004

Rights Holder(s)

Scopus

Bibliographic Citation

Electronic Journal of Biotechnology. Vol 13, No.5 (2010), p.-

Suggested Citation

Phonnok S., Tanechpongtamb W.U., Wongsatayanon B.T. Anticancer and apoptosis-inducing activities of microbial metabolites. Electronic Journal of Biotechnology. Vol 13, No.5 (2010), p.-. doi:10.2225/vol13-issue5-fulltext-7 Retrieved from: https://hdl.handle.net/20.500.14740/7453

Title

Anticancer and apoptosis-inducing activities of microbial metabolites

Author(s)

Phonnok S.
 Tanechpongtamb W.U.
 Wongsatayanon B.T.

Abstract

The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were observed in all treated cancer cells. DNA-binding dye staining demonstrated nuclear condensation and fragmentation. Chromosomal DNA cleavage detected as DNA ladder pattern by gel electrophoresis including activation of cellular caspase-3 activity, a hallmark of apoptosis, were observed in all treated cancer cell lines. These characteristics suggested the mechanism of apoptosis cell death induced by the extracts. No growth inhibition and apoptosis characteristic were detected in normal cells even at high concentration used suggesting the selective cytotoxicity and potential candidates to develop as anticancer agents. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile.

Subject(s)

Anti-cancer agents
 Anti-proliferative
 Anticancer
 Anticancer activities
 Apoptosis-inducing activity
 Bioactive compounds
 Cancer cell lines
 Cancer cells
 Cancer Chemotherapy
 Caspase-3
 Caspases
 Cell extracts
 Cell-shrinkage
 DNA cleavage
 DNA ladder
 DNA-binding
 Drug resistance
 Gel electrophoresis
 Growth inhibition
 High concentration
 Microbial products
 Morphological changes
 MTT assays
 Surface contact
 Cell culture
 Chemotherapy
 Diseases
 DNA
 Electrophoresis
 Lead compounds
 Metabolism
 Metabolites
 Cell death
 Caspase 3
 DNA
 Doxorubicin
 Microbial products not classified elsewhere
 Acinetobacter baumannii
 Animal cell
 Antineoplastic activity
 Apoptosis
 Article
 Bacillus
 Cancer cell culture
 Cancer inhibition
 Candida tropicalis
 Cell proliferation
 Cell structure
 Concentration response
 Controlled study
 DNA cleavage
 DNA fragmentation
 Drug cytotoxicity
 Drug isolation
 Drug potency
 Drug screening
 Drug specificity
 Enzyme activation
 IC 50
 Nonhuman
 Pseudomonas aeruginosa

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URI

https://hdl.handle.net/20.500.14740/7453

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Scopus: Year 1983-2021

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