Publication: The rapid detection method by polymerase chain reaction for minute intestinal trematodes: Haplorchis taichui in intermediate snail hosts based on 18s ribosomal DNA
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Issued Date
2018
Resource Type
File Type
application/pdf
ISSN
9717196
Other identifier(s)
2-s2.0-85050596156
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Parasitic Diseases. Vol 42, No.3 (2018), p.423-432
Suggested Citation
Chontananarth T., Anucherngchai S., Tejangkura T. The rapid detection method by polymerase chain reaction for minute intestinal trematodes: Haplorchis taichui in intermediate snail hosts based on 18s ribosomal DNA. Journal of Parasitic Diseases. Vol 42, No.3 (2018), p.423-432. doi:10.1007/s12639-018-1020-0 Retrieved from: https://hdl.handle.net/20.500.14740/5866
Author(s)
Abstract
The minute intestinal trematode, Haplorchis taichui, is an important parasite species that can infect humans and other mammals. This study investigated the outbreak of H. taichui in thiarid snails in the lower part of the Chao Phraya Basin, Thailand by employing morphological and molecular-based methods. In development of a specific primer of H. taichui, the PCR reaction was conducted with no cross-reaction to their hosts and other related trematode species. The highest level of sensitivity that could be amplified was 0.50 ng/μl and this was detected with only one egg in the sample. In terms of the epidemic results, the parapleurolophocercous cercaria infected only two species of thiarid snails (Melanoides tuberculata and Tarebia granifera) with an overall prevalence of 3.80% (23/605). The process of molecular identification revealed positive results indicating that eleven from twenty-three of parapleurolophocercous cercariae specimens in the lower part of the Chao Phraya Basin were H. taichui. In conclusion, this study has developed a rapid detection method, which can discriminate H. taichui from other parapleurolophocercous cercaria in intermediate snail hosts with a high level of sensitivity. Moreover, the high proportion of H. taichui in parapleurolophocercous cercaria (47.83%) indicated that H. taichui was the dominant species of this cercarial type and could infect cyprinoid fish in the lower part of the Chao Phraya Basin leading to public health problems in this area. Thus, a specific primer could be useful in the detection and surveillance of H. taichui outbreaks in their hosts. Recognition of this has resulted in the creation of important prevention programs in these infected areas in the further study. © 2018, Indian Society for Parasitology.
Subject(s)
DNA 18S
Genomic DNA
Adult
Agar gel electrophoresis
Animal cell
Animal experiment
Animal model
Animal tissue
Article
Controlled study
Disease surveillance
DNA sequence
Geographic distribution
Geographic information system
Haplorchis taichui
Intestine infection
Melanoides tuberculata
Molecular diagnosis
Nonhuman
Parapleurolophocercous cercaria
Parasite identification
Polymerase chain reaction
Prevalence
Priority journal
Sensitivity and specificity
Snail
Tarebia granifera
Trematodiasis
Genomic DNA
Adult
Agar gel electrophoresis
Animal cell
Animal experiment
Animal model
Animal tissue
Article
Controlled study
Disease surveillance
DNA sequence
Geographic distribution
Geographic information system
Haplorchis taichui
Intestine infection
Melanoides tuberculata
Molecular diagnosis
Nonhuman
Parapleurolophocercous cercaria
Parasite identification
Polymerase chain reaction
Prevalence
Priority journal
Sensitivity and specificity
Snail
Tarebia granifera
Trematodiasis