Publication:
Characterization of flagellotropic, chi-like salmonella phages isolated from thai poultry farms

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

19994915

DOI

10.3390/v11060520

Other identifier(s)

2-s2.0-85068166916

Rights Holder(s)

Scopus

Bibliographic Citation

Viruses. Vol 11, No.6 (2019)

Suggested Citation

Phothaworn P., Dunne M., Supokaivanich R., Ong C., Lim J., Taharnklaew R., Vesaratchavest M., Khumthong R., Pringsulaka O., Ajawatanawong P., Klumpp J., Brown N., Imam M., Clokie M.R.J., Galyov E.E., Korbsrisate S. Characterization of flagellotropic, chi-like salmonella phages isolated from thai poultry farms. Viruses. Vol 11, No.6 (2019). doi:10.3390/v11060520 Retrieved from: https://hdl.handle.net/20.500.14740/5329

Title

Characterization of flagellotropic, chi-like salmonella phages isolated from thai poultry farms

Author(s)

Phothaworn P.
 Dunne M.
 Supokaivanich R.
 Ong C.
 Lim J.
 Taharnklaew R.
 Vesaratchavest M.
 Khumthong R.
 Pringsulaka O.
 Ajawatanawong P.
 Klumpp J.
 Brown N.
 Imam M.
 Clokie M.R.J.
 Galyov E.E.
 Korbsrisate S.

Abstract

Despite a wealth of knowledge on Salmonella phages worldwide, little is known about poultry-associated Salmonella phages from Thailand. Here, we isolated 108 phages from Thai poultry farms that infect Salmonella enterica serovar Typhimurium. Phages STm101 and STm118 were identified as temperate Siphoviridae phages. Genome sequencing and analyses revealed these phages share approximately 96% nucleotide sequence similarity to phage SPN19, a member of the Chi-like virus genus. PCR amplification of the gene encoding capsid protein E of the Chi-like phage was positive for 50% of phage isolates, suggesting a predominance of this phage type among the sampled poultry farms. In addition to the flagella, two phages required the lipopolysaccharide to infect and lyse Salmonella. Furthermore, phylogenomic analysis demonstrated that phages STm101 and STm118 formed a monophyletic clade with phages isolated from Western countries, but not from closer isolated phages from Korea. However, further investigation and more phage isolates are required to investigate possible causes for this geographic distribution. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Subject(s)

Bacterium lipopolysaccharide
 Capsid protein
 Agricultural land
 Article
 Bacterial genome
 Bacterium culture
 DNA base composition
 Escherichia coli
 Flagellum
 Gene amplification
 Gene sequence
 Genetic analysis
 Host range
 Listeria monocytogenes
 Next generation sequencing
 Nonhuman
 Nucleotide sequence
 Phage titer
 Phylogenomics
 Poultry
 Salmonella enterica serovar Agona
 Salmonella enterica serovar Albany
 Salmonella enterica serovar Altona
 Salmonella enterica serovar Choleraesuis
 Salmonella enterica serovar Corvalis
 Salmonella enterica serovar Enteritidis
 Salmonella enterica serovar Hadar
 Salmonella enterica serovar Kentucky
 Salmonella enterica serovar Saintpaul
 Salmonella enterica serovar Schwarzengrund
 Salmonella enterica serovar Singapore
 Salmonella enterica serovar Typhimurium
 Salmonella enterica serovar Virchow
 Salmonella phage
 Staphylococcus aureus
 Thai (citizen)
 Thailand
 Transmission electron microscopy
 Vibrio
 Animal
 Classification
 Genetics
 Isolation and purification
 Microbiology
 Phylogeny
 Phylogeography
 Siphoviridae
 Virology
 Virus genome
 Animals
 Farms
 Genome, Viral
 Phylogeny
 Phylogeography
 Poultry
 Salmonella Phages
 Salmonella typhimurium
 Siphoviridae
 Thailand

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URI

https://hdl.handle.net/20.500.14740/5329

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Scopus: Year 1983-2021

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