Publication:
Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1775103

DOI

10.3354/dao02482

Other identifier(s)

2-s2.0-84866232879

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Diseases of Aquatic Organisms. Vol 99, No.3 (2012), p.197-205

Suggested Citation

Longyant S., Senapin S., Sanont S., Wangman P., Chaivisuthangkura P., Rukpratanporn S., Sithigorngul P. Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus. Diseases of Aquatic Organisms. Vol 99, No.3 (2012), p.197-205. doi:10.3354/dao02482 Retrieved from: https://hdl.handle.net/20.500.14740/7000

Title

Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus

Author(s)

Longyant S.
 Senapin S.
 Sanont S.
 Wangman P.
 Chaivisuthangkura P.
 Rukpratanporn S.
 Sithigorngul P.

Abstract

The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S- ENGLtransferase (GST)-ENGLfusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot, and immunohistochemistry (IHC) methods, 4 MAbs specific to the XSV CP detected XSV in the freshwater prawn Macrobrachium rosenbergii without crossreaction to host proteins or to proteins of Macrobrachium rosenbergii nodavirus (MrNV) or 5 of the most pathogenic viruses of penaeid shrimp. In dot blots, the combined MAbs could detect down to ~10 to 20 fmol μl-1 of purified GST-XCP protein, which was somewhat more sensitive compared to any single MAb. Used in conjunction with an MrNV-specific MAb, white tail disease (WTD) was diagnosed more effectively. However, the sensitivity at which the combined 4 MAbs detected XSV CP was 1000-fold lower than XSV RNA detected by RT-PCR. IHC analysis of M. rosenbergii tissue sections using the MAbs showed XSV infection to co-localize at variable loads with MrNV infection in heart and muscle cells as well as cells of connective tissues in the hepatopancreas. Since XSV histopathology remained prominent in tissues of some prawns in which MAb reactivity for MrNV was low compared to MAb reactivity for XSV, XSV might play some role in WTD severity. © Inter-Research 2012.

Subject(s)

Monoclonal antibody
 Virus RNA
 Antibody
 Electrokinesis
 Enzyme activity
 Immunoassay
 Muscle
 Pathogenicity
 Prawn culture
 Protein
 Viral disease
 Animal
 Article
 Hybridoma
 Immunology
 Mouse
 Nodavirus
 Palaemonidae
 Physiology
 Virology
 Animals
 Antibodies, Monoclonal
 Hybridomas
 Mice
 Nodaviridae
 Palaemonidae
 RNA, Viral
 Decapoda (Crustacea)
 Escherichia coli
 Macrobrachium rosenbergii
 Macrobrachium rosenbergii nodavirus
 Miridae
 Mus
 Penaeidae
 White tip die-back phytoplasma

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URI

https://hdl.handle.net/20.500.14740/7000

Collections

Scopus: Year 1983-2021

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