Publication:
Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes

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Issued Date

2007

Resource Type

Article

File Type

application/pdf

ISSN

10313613

DOI

10.1071/RD06097

Other identifier(s)

2-s2.0-33846630322

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Reproduction, Fertility and Development. Vol 19, No.2 (2007), p.383-391

Suggested Citation

Boonkusol D., Faisaikarm T., Dinnyes A., Kitiyanant Y. Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes. Reproduction, Fertility and Development. Vol 19, No.2 (2007), p.383-391. doi:10.1071/RD06097 Retrieved from: https://hdl.handle.net/20.500.14740/4821

Title

Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes

Author(s)

Boonkusol D.
 Faisaikarm T.
 Dinnyes A.
 Kitiyanant Y.

Abstract

The purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. In vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (SSV) and in-straw vitrification (ISV), respectively. Survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida intactness, as well as parthenogenetic blastocyst rates of vitrified-warmed oocytes were significantly higher with SSV (89.3 and 13.6%, respectively) than ISV (81.8 and 5.5%, respectively). However, they were still significantly lower than that of control oocytes (100 and 34.2%, respectively). For examining the ultrastructural changes, fresh, VS-exposed (ISV and SSV), and vitrified-warmed (ISV and SSV) oocytes were processed for transmission electron microscopy. In VS-exposed oocytes, reduction of microvilli abundance and damage of mitochondrial membrane were found only in the ISV group. In vitrified-warmed oocytes, however, it was clear that both methods of vitrification induced profound ultrastructural modifications to microvilli, mitochondria, oolemma and cortical granules as well as to the size and position of vesicles. Damaged mitochondria were, however, more abundant in ISV vitrified oocytes than in SSV vitrified oocytes, which correlated with the developmental data, showing the superiority of the SSV method. The present study demonstrated the feasibility of vitrification of in vitro-matured swamp buffalo oocytes. © CSIRO 2007.

Subject(s)

Ethylene glycol
 Animal cell
 Article
 Blastocyst
 Buffalo
 Cell survival
 Cell ultrastructure
 Cell vacuole
 Controlled study
 Correlation analysis
 Cryopreservation
 Feasibility study
 Membrane damage
 Microvillus
 Mitochondrial membrane
 Mitochondrion
 Nonhuman
 Oocyte
 Oocyte development
 Oocyte maturation
 Survival rate
 Transmission electron microscopy
 Vitrification
 Zona pellucida
 Animals
 Buffaloes
 Cell Survival
 Cryopreservation
 Ethylene Glycol
 Female
 Microscopy, Electron
 Mitochondria
 Oocytes
 Surface Properties
 Bubalus bubalis
 Sugarcane streak virus

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URI

https://hdl.handle.net/20.500.14740/4821

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Scopus: Year 1983-2021

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