Publication: Simple and rapid detection of infectious myonecrosis virus using an immunochromatographic strip test
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0
Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
3048608
Other identifier(s)
2-s2.0-84883295294
Rights Holder(s)
Scopus
Bibliographic Citation
Archives of Virology. Vol 158, No.9 (2013), p.1925-1930
Suggested Citation
Chaivisuthangkura P., Senapin S., Wangman P., Longyant S., Sithigorngul P. Simple and rapid detection of infectious myonecrosis virus using an immunochromatographic strip test. Archives of Virology. Vol 158, No.9 (2013), p.1925-1930. doi:10.1007/s00705-013-1680-0 Retrieved from: https://hdl.handle.net/20.500.14740/6562
Abstract
A strip test was developed for detection of infectious myonecrosis virus (IMNV) using a pair of monoclonal antibodies (MAbs), called IMN7 and IMC6, that are specific for the N and C fragments, respectively, of the IMNV capsid protein. The test strips were placed in plastic cassettes and stored desiccated in sealed plastic bags. In detection assays using the test-strip cassettes, 100-μl samples of application buffer containing homogenates from muscles or pleopods of normal or IMNV-infected shrimp were applied to the cassette sample chamber. Subsequent flow through the glass-fiber pad and the nitrocellulose membrane strip led to the development of visible antibody-protein complexes within 15 min. In samples containing IMNV, viral capsid protein bound to gold-labeled IMN7 in the glass-fiber pad and the complex was subsequently captured by MAb IMC6 at the T line to form a reddish-purple band. Any unbound gold-labeled IMN7 migrated past the T line to be captured by the GAM antibody to form a band at the C line. Samples without IMNV or containing it below the test detection limit gave reddish-purple bands only at the C line. The sensitivity of the test was comparable to that of dot blot tests using single MAbs but was ~300-fold less sensitive than a one-step RT-PCR test for IMNV. Despite this lower sensitivity, the strip test has advantages of low cost, speed and simplicity (i.e., no sophisticated equipment or specialized skills required), and it is appropriate for use by farmers for pathogen confirmation when IMNV is suspected in diseased shrimp. © 2013 Springer-Verlag Wien.
Subject(s)
Capsid protein
Colloidal gold
Monoclonal antibody
Virus antibody
Animal
Article
Chemistry
Evaluation
Genetics
Immunoaffinity chromatography
Immunology
Isolation and purification
Methodology
Penaeidae
Reverse transcription polymerase chain reaction
RNA virus
Sensitivity and specificity
Time
Virology
Animals
Antibodies, Monoclonal
Antibodies, Viral
Capsid Proteins
Gold Colloid
Immunochromatography
Penaeidae
Reverse Transcriptase Polymerase Chain Reaction
RNA Viruses
Sensitivity and Specificity
Time Factors
Decapoda (Crustacea)
Miridae
Colloidal gold
Monoclonal antibody
Virus antibody
Animal
Article
Chemistry
Evaluation
Genetics
Immunoaffinity chromatography
Immunology
Isolation and purification
Methodology
Penaeidae
Reverse transcription polymerase chain reaction
RNA virus
Sensitivity and specificity
Time
Virology
Animals
Antibodies, Monoclonal
Antibodies, Viral
Capsid Proteins
Gold Colloid
Immunochromatography
Penaeidae
Reverse Transcriptase Polymerase Chain Reaction
RNA Viruses
Sensitivity and Specificity
Time Factors
Decapoda (Crustacea)
Miridae