Publication:
Soy isoflavones improves endometrial barrier through tight junction gene expression

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

14701626

DOI

10.1530/REP-14-0269

Other identifier(s)

2-s2.0-84922355352

Rights Holder(s)

Scopus

Bibliographic Citation

Reproduction. Vol 149, No.3 (2015), p.269-280

Suggested Citation

Kiatprasert P., Deachapunya C., Benjanirat C., Poonyachoti S. Soy isoflavones improves endometrial barrier through tight junction gene expression. Reproduction. Vol 149, No.3 (2015), p.269-280. doi:10.1530/REP-14-0269 Retrieved from: https://hdl.handle.net/20.500.14740/6169

Title

Soy isoflavones improves endometrial barrier through tight junction gene expression

Author(s)

Kiatprasert P.
 Deachapunya C.
 Benjanirat C.
 Poonyachoti S.

Abstract

Contamination with bacterial endotoxin causes the disruption of the tight junction (TJ) barrier. We investigated the ameliorative effect of dietary flavonoids genistein (Ge) and daidzein (Di) in normal or lipopolysaccharide (LPS)-induced disruption of epithelial barrier function of the endometrium. Using the immortalized porcine glandular endometrial epithelial cells (PEG), transepithelial electrical resistance (TER) and FITC-dextran flux (FD-4) across the monolayer were measured. The mRNA expression of TJ proteins, zona occludens-1 (ZO1), and claudin-1, -3, -4, -7 and -8 was evaluated by real-time RT-PCR for coinciding effect of Ge or Di occurred at the gene transcription level. The results revealed that Ge and Di altered the TER, depending on times and concentrations. Low concentration (10K10 M) of both compounds decreased the TER, whereas higher concentrations (10-8 and 10-6 M) increased the TER which was not related to the FD-4 flux. The increased TER by Ge or Di was parallel to the induction of claudin-3 and -4 or -8 mRNA expression respectively. With LPS inoculation, all isoflavone treatments inhibited the decreased TER induced by LPS, but only Ge (10-8 or 10-6 M) or Di (10-10 or 10-6 M) was coincidence with the decreased FD-4 flux. Under this LPS-stimulated condition, some or all examined TJ gene expressions appeared to be promoted by specific concentration of Ge or Di respectively. Our findings suggest that the soy isoflavones treatment could promote and restore the impaired endometrial barrier function caused by LPS contamination. © 2015 Society for Reproduction and Fertility.

Subject(s)

Claudin 1
 Claudin 3
 Claudin 4
 Claudin 7
 Claudin 8
 Daidzein
 Endotoxin
 Fluorescein isothiocyanate dextran
 Genistein
 Isoflavone derivative
 Lipopolysaccharide
 Messenger RNA
 Protein ZO1
 Tight junction protein
 Claudin
 Genistein
 Isoflavone derivative
 Lipopolysaccharide
 Phytoestrogen
 Protein ZO1
 Animal cell
 Article
 Concentration response
 Controlled study
 Electric resistance
 Endometrium
 Female
 Gene expression
 Genetic transcription
 Monolayer culture
 Nonhuman
 Priority journal
 Real time polymerase chain reaction
 Soybean
 Swine
 Tight junction
 Transepithelial electrical resistance
 Animal
 Cell line
 Drug effects
 Endometrium
 Gene expression
 Genetics
 Metabolism
 Pig
 Tight junction
 Bacteria (microorganisms)
 Sus
 Animals
 Cell Line
 Claudins
 Endometrium
 Female
 Gene Expression
 Genistein
 Isoflavones
 Lipopolysaccharides
 Phytoestrogens
 Swine
 Tight Junctions
 Zonula Occludens-1 Protein

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URI

https://hdl.handle.net/20.500.14740/6169

Collections

Scopus: Year 1983-2021

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