Publication:
Purification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii

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Issued Date

1999

Resource Type

Article

File Type

application/pdf

ISSN

0022104X

DOI

10.1002/(SICI)1097-010X(19990701)284:2<217

Other identifier(s)

2-s2.0-0033168282

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Experimental Zoology. Vol 284, No.2 (1999), p.217-224

Suggested Citation

Sithigorngul W., Jaideechoey S., Saraithongkum W., Longyant S., Sithigorngul P. Purification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii. Journal of Experimental Zoology. Vol 284, No.2 (1999), p.217-224. doi:10.1002/(SICI)1097-010X(19990701)284:2<217 Retrieved from: https://hdl.handle.net/20.500.14740/7129

Title

Purification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii

Author(s)

Sithigorngul W.
 Jaideechoey S.
 Saraithongkum W.
 Longyant S.
 Sithigorngul P.

Abstract

Isolation of the crustacean hyperglycemic hormone (CHH) from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii was performed using 5,000 ground eye-stalks extracted in methanol-acetic acid-water (90:1:9). After the extract was partially purified using C18 cartridges, it was further purified by eight steps of RP-HPLC using four kinds of columns: C18, C8, cyano and phenyl, and three solvent systems: acetonitrile (ACN)/trifluoroacetic acid, ACN/heptafluoroacetic acid and ACN/triethylammonium acetate. The bioassay of CHH during purification was done by injection of eluate fractions into eyestalk-ablated prawns and determination of the ability of the fractions to elevate glucose in the haemolymph. A complete amino acid sequence analysis was performed on one isoform of CHH (Mar-CHH-1), consisting of 71 residues. The sequence of Mar-CHH-1 shows considerable similarity (45-68%) to CHHs reported in other crustaceans. It is expected that there might be more than one isoform of CHH in M. rosenbergii.

Subject(s)

Acetonitrile
 Amino acid sequence
 Bioassay
 Crustacean hyperglycemic hormone
 Macrobrachium rosenbergii
 Purification
 Reversed phase high performance liquid chromatography
 Solvent extraction
 Trifluoroacetic acid
 Amino Acid Sequence
 Animals
 Chromatography, High Pressure Liquid
 Eye
 Female
 Ganglia, Invertebrate
 Glucose
 Hemolymph
 Hyperglycemia
 Invertebrate Hormones
 Molecular Sequence Data
 Palaemonidae
 Protein Isoforms
 Sequence Homology, Amino Acid
 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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URI

https://hdl.handle.net/20.500.14740/7129

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Scopus: Year 1983-2021

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