Publication:
Assessing the Expression of Aquaporin 3 Antigen-Recognition Sites in Oral Squamous Cell Carcinoma

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

15412016

DOI

10.1097/PAI.0000000000000802

Other identifier(s)

2-s2.0-85090613971

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Applied Immunohistochemistry and Molecular Morphology. Vol 28, No.8 (2020), p.611-620

Suggested Citation

Udompatanakorn C., Yada N., Matsuo K. Assessing the Expression of Aquaporin 3 Antigen-Recognition Sites in Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology. Vol 28, No.8 (2020), p.611-620. doi:10.1097/PAI.0000000000000802 Retrieved from: https://hdl.handle.net/20.500.14740/4426

Title

Assessing the Expression of Aquaporin 3 Antigen-Recognition Sites in Oral Squamous Cell Carcinoma

Author(s)

Udompatanakorn C.
 Yada N.
 Matsuo K.

Abstract

Aquaporin 3 (AQP3) serves as a water and glycerol transporter facilitating epithelial cell hydration. Recently, the involvement of AQP3 in cancers has been reported. However, the immunohistochemical expression of AQP3 in carcinomas remains controversial. We hypothesized that differences in aquaporin 3 antigen recognition (AQP3 AR) may influence their expressions. Thus, our study aimed to assess the immunostaining patterns of 3 AQP3 AR sites in oral squamous cell carcinoma (OSCC) and to compare the adjacent areas of high-grade epithelial dysplasia (HG-ED) and normal oral mucosa (NOM). The study group included formalin-fixed OSCC samples (n=51) with adjacent regions of HG-ED (n=12) and NOM (n=51). The tissues were stained with anti-AQP3 antibodies (AR sites at amino acid (AA) 250-C terminus, AA180-228, and N terminus AA1-80) by immunohistochemistry. Our results showed that strong membranous immunostaining was observed for AQP3 AR sites at the AA250-C terminus and AA180-228 in all the samples for NOM and weak AQP3 immunostaining for both the AR sites in all the 12 samples for HG-ED. The invasive front of OSCC samples showed that AQP3 AR at the AA250-C terminus decreased in 42/51 samples (82.4%) and AA180-228 in 47/51 samples (92.2%). Conversely, in the AQP3 AR site at N terminus AA1-80, all samples of the NOM showed negative or slightly positive staining in the cytoplasm of the lower layers. AQP3 expression was increased in 12/12 cases (100%) and 46/51 cases (90.2%) in the HG-ED and invasive front of OSCC, respectively. AQP3 may be used as a biomarker for detecting malignant transformations. AQP3 AR site differences influence their immunohistochemical expression in OSCC. © 2020 Lippincott Williams and Wilkins. All rights reserved.

Subject(s)

Anti aquaporin 3 antibody
 Aquaporin 3
 Aquaporin antigen recognition
 Formaldehyde
 Glycerol
 Membrane protein
 Unclassified drug
 AA1 80
 AA180 228
 AA250 c terminus
 Adult
 Aged
 Amino terminal sequence
 Antigen recognition
 Article
 Cancer staging
 Carboxy terminal sequence
 Controlled study
 Dysplasia
 Female
 Gingiva
 High grade epithelial dysplasia
 Human
 Human tissue
 Hydration
 Immunohistochemistry
 Lymph node metastasis
 Male
 Mouth cavity
 Mouth floor
 Mouth mucosa
 Mouth squamous cell carcinoma
 Paraffin embedding
 Priority journal
 Tongue

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URI

https://hdl.handle.net/20.500.14740/4426

Collections

Scopus: Year 1983-2021

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