Specific primers of oryzias javanicus, O. dancena, O. minutillus and O. woworae and molecular phylogeny using partial mitochondrial DNA sequences

Abstract

Fish of the genus Oryzias (Teleost) are one of the experimental animals used in various studies of biological vertebrates. These fish mostly inhabit the fresh and brackish waters of East and Southeast Asia. In the present study, specific primer pairs were used to identify the four species of Oryzias (O. javanicus, O. dancena, O. minutillus and O. woworae) using PCR (polymerase chain reaction) analysis. Moreover, the phylogenetic relationship of O. woworae within the eleven Oryzias species was assessed based on the complete nucleotide sequence of tDNA-Val and the partial sequences 12S ribosomal DNA (rDNA) and 16S rDNA. The PCR results showed individual amplification by the specific primers in the four species. The phylogenetic data, which clustered into three groups, confirmed the hypothesis that the Oryzias taxa referred to those sequences. These data give a set of molecular tools and a source of nucleotide data for Oryzias that might increase the knowledge distribution of molecular genetics in the Adrianichthyidae. © 2014 Academic Journals Inc.