Publication:
Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction

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Issued Date

1998

Resource Type

Article

File Type

application/pdf

ISSN

3044017

DOI

10.1016/S0304-4017(98)00145-9

Other identifier(s)

2-s2.0-0032584962

Rights Holder(s)

Scopus

Bibliographic Citation

Veterinary Parasitology. Vol 78, No.4 (1998), p.259-264

Suggested Citation

Watanapokasin Y., Tananyutthawongese C., Uthaisang W., Chansiri K., Boonmatit C., Sarataphan N. Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction. Veterinary Parasitology. Vol 78, No.4 (1998), p.259-264. doi:10.1016/S0304-4017(98)00145-9 Retrieved from: https://hdl.handle.net/20.500.14740/7199

Title

Intra-species differentiation of Trypanosoma evansi by DNA fingerprinting with arbitrary primered polymerase chain reaction

Author(s)

Watanapokasin Y.
 Tananyutthawongese C.
 Uthaisang W.
 Chansiri K.
 Boonmatit C.
 Sarataphan N.

Abstract

A method was developed to obtain reproducible DNA fingerprints from isolates of Trypanosoma evansi by PCR-based amplification using arbitrary primers (AP-PCR). Only one out of 10 randomly designed 12-mer primers generated DNA fingerprint profiles that revealed intra-species differences in T. evansi. The technique was applied in association with parasitological and serological examinations to investigate animal-to-animal transmission during an outbreak of surra in Thailand. The AP-PCR method has the advantage of being simple, fast and sensitive to diagnosis and characterization of the parasites since it does not require prior DNA sequence information. The technique should prove useful for the proper understanding of epidemiology and for designing rational control programs for trypanosomosis. Copyright (C) 1998 Elsevier Science B.V.

Subject(s)

Animal experiment
 Animal model
 Article
 Dna fingerprinting
 Gene amplification
 Mouse
 Nonhuman
 Parasite identification
 Polymerase chain reaction
 Species differentiation
 Trypanosoma
 Trypanosomiasis
 Animals
 Cattle
 Disease Outbreaks
 DNA Fingerprinting
 DNA Primers
 Mice
 Polymerase Chain Reaction
 Thailand
 Trypanosoma
 Trypanosomiasis, Bovine
 Animalia
 Trypanosoma
 Trypanosoma evansi

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URI

https://hdl.handle.net/20.500.14740/7199

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Scopus: Year 1983-2021

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