Publication:
Cercarial trematodes in freshwater snails from Bangkok, Thailand: Prevalence, morphological and molecular studies and human parasite perspective

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Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

311820

DOI

10.1017/S0031182020002073

Other identifier(s)

2-s2.0-85095816795

Rights Holder(s)

Scopus

Bibliographic Citation

Parasitology. Vol 148, No.3 (2021), p.366-383

Suggested Citation

Wiroonpan P., Chontananarth T., Purivirojkul W. Cercarial trematodes in freshwater snails from Bangkok, Thailand: Prevalence, morphological and molecular studies and human parasite perspective. Parasitology. Vol 148, No.3 (2021), p.366-383. doi:10.1017/S0031182020002073 Retrieved from: https://hdl.handle.net/20.500.14740/7870

Title

Cercarial trematodes in freshwater snails from Bangkok, Thailand: Prevalence, morphological and molecular studies and human parasite perspective

Author(s)

Wiroonpan P.
 Chontananarth T.
 Purivirojkul W.

Abstract

We investigated the prevalence, morphological characters and molecular classifications of trematode cercariae in freshwater snails randomly collected from 59 sampling localities in Bangkok from May 2018 to March 2019. We used a crushing technique to observe the cercarial stage inside each snail body and amplified the internal transcribed spacer 2 regions of cercarial DNA using polymerase chain reaction methodology. The associated phylogenetic tree was reconstructed using Bayesian inference analyses. A total of 517 of 15 621 examined snails were infected with trematode cercariae, and the infected snails were classified into 11 species of seven families with a 3.31% overall prevalence of the infection. The Bithynia siamensis siamensis snail displayed the highest prevalence of infection (16.16%), whereas the Physella acuta snail exhibited the lowest prevalence (0.08%) of infection. Eight morphological types of cercariae were observed. The highest prevalence of infection was observed in mutabile cercaria (1.86%). Based on molecular investigations, the phylogram revealed eight cercaria types assigned to at least nine digenean trematode families, of which five belong to groups of human intestinal flukes. Although, with the exception of schistosome cercaria, trematode cercariae are not known to directly damage humans, understanding the general biology of trematode cercariae (including diversity, distribution, infection rates and host range) is important and necessary for the prevention and control of parasitic transmission that impacts aquatic cultivations, livestock farming and human health. Copyright © The Author(s), 2020. Published by Cambridge University Press.

Subject(s)

Fresh water
 Genomic DNA
 Internal transcribed spacer 2
 Fresh water
 Article
 Bithynia
 Bithynia siamensis siamensis
 Body build
 Cercaria
 Controlled study
 Digenea
 DNA extraction
 DNA purification
 DNA sequencing
 Echinostoma caproni
 Echinostoma revolutum
 Echinostomatidae
 Gene amplification
 Human
 Infection rate
 Morphological trait
 Nonhuman
 Nucleotide sequence
 Phylogenetic tree
 Physella acuta
 Polymerase chain reaction
 Prevalence
 Prevention and control
 Priority journal
 Schistosoma
 Thailand
 Trematode
 Trematodiasis
 Unindexed sequence
 Anatomy and histology
 Animal
 Classification
 Genetics
 Growth, development and aging
 Host parasite interaction
 Parasitology
 Population dynamics
 Snail
 Animals
 Cercaria
 Fresh Water
 Host-Parasite Interactions
 Population Dynamics
 Snails
 Thailand
 Trematoda

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URI

https://hdl.handle.net/20.500.14740/7870

Collections

Scopus: Year 1983-2021

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