Publication:
Experimental infections reveal that common Thai crustaceans are potential carriers for spread of exotic Taura syndrome virus

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

1775103

DOI

10.3354/dao01903

Other identifier(s)

2-s2.0-44949127940

Rights Holder(s)

Scopus

Bibliographic Citation

Diseases of Aquatic Organisms. Vol 79, No.3 (2008), p.183-190

Suggested Citation

Kiatpathomchai W., Jaroenram W., Arunrut N., Gangnonngiw W., Boonyawiwat V., Sithigorngul P. Experimental infections reveal that common Thai crustaceans are potential carriers for spread of exotic Taura syndrome virus. Diseases of Aquatic Organisms. Vol 79, No.3 (2008), p.183-190. doi:10.3354/dao01903 Retrieved from: https://hdl.handle.net/20.500.14740/4032

Title

Experimental infections reveal that common Thai crustaceans are potential carriers for spread of exotic Taura syndrome virus

Author(s)

Kiatpathomchai W.
 Jaroenram W.
 Arunrut N.
 Gangnonngiw W.
 Boonyawiwat V.
 Sithigorngul P.

Abstract

Taura syndrome virus (TSV) was first reported as a serious cause of shrimp mortality limited to reared Penaeus (Litopenaeus) vannamei in the Americas, where it spread principally through regional and international transfer of live post larvae (PL) and broodstock. Subsequently, through importation of infected broodstock, TSV outbreaks spread to Asia, first to Taiwan and China and then to Thailand, Indonesia and Korea. Since its introduction to Thailand, outbreaks have occasionally been reported from rearing ponds stocked with batches of specific pathogen free (SPF) P. vannamei PL that tested negative for TSV by nested RT-PCR assay. Since it was possible that the outbreaks may have occurred via horizontal transfer of TSV from wild carrier species, we tested 5 common native crustaceans that live in and around shrimp ponds (2 palaemonid shrimp species, Palaemon styliferus and Macrobrachium lanchesteri, and 3 species of crabs, Sesarma mederi, Scylla serrata and Uca vocans) for susceptibility to TSV in experimental challenges. We found that U. vocans, S. serrata and S. mederi did not die but, respectively, gave strong RT-PCR reactions indicating heavy viral load at 5 , 10 and 15 d post-injection of TSV and 10, 15 and up to 50 d after feeding with TSV-infected P. vannamei carcasses. Also after feeding, P. styliferus did not die, but a high proportion gave strong RT-PCR reactions at 5 d post-challenge and no reactions at 15 d. Similarly after feeding, M. lanchesteri showed no mortality and gave only light RT-PCR reactions at 2 d, moderate reactions at 5 d and no reaction at 15 d. By contrast, transmission experiments from the TSV-infected crabs and palaemonid shrimp via water or feeding resulted in death of all the exposed P. vannamei from 8 to 12 d post-challenge and all were positive for heavy viral load by RT-PCR assay. Despite the results of these laboratory challenge tests, natural TSV infections were not detected by nested RT-PCR in samples of these species taken from the wild. These results indicated that transmission of TSV from infected crabs and palaemonid shrimp via water or feeding might pose a potential risk to shrimp aquaculture. © Inter-Research 2008.

Subject(s)

Virus RNA
 Brood stock
 Crab
 Crustacean
 Disease spread
 Experimental design
 Feeding
 Infectious disease
 Mortality
 Pathogen
 Shrimp culture
 Viral disease
 Animal
 Aquaculture
 Article
 Brachyura
 Genetics
 Isolation and purification
 Palaemonidae
 Picornavirus
 Thailand
 Virology
 Animals
 Aquaculture
 Brachyura
 Palaemonidae
 Picornaviridae
 RNA, Viral
 Thailand
 Crustacea
 Decapoda (Crustacea)
 Exopalaemon styliferus
 Litopenaeus vannamei
 Macrobrachium lanchesteri
 Palaemonidae
 Scylla serrata
 Sesarma
 Taura syndrome virus
 Thalassuca vocans

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URI

https://hdl.handle.net/20.500.14740/4032

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Scopus: Year 1983-2021

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