Publication: Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells
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Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
1021335X
Other identifier(s)
2-s2.0-84964053957
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Oncology Reports. Vol 35, No.5 (2016), p.2851-2858
Suggested Citation
Innajak S., Mahabusrakum W., Watanapokasin R. Goniothalamin induces apoptosis associated with autophagy activation through MAPK signaling in SK-BR-3 cells. Oncology Reports. Vol 35, No.5 (2016), p.2851-2858. doi:10.3892/or.2016.4655 Retrieved from: https://hdl.handle.net/20.500.14740/5447
Author(s)
Abstract
Goniothalamin, a plant bioactive styrly-lactone, possesses many biological activities. In the present study, the anticancer effect of goniothalamin on human breast cancer cell line SK-BR-3 was investigated. The results showed that goniothalamin induced nuclear condensation, DNA fragmentation, apoptotic bodies and mitochondrial dysfunction as determined by JC-1 staining. Goniothalamin also increased the Bax/Bcl-2 ratio and expression of cleaved caspase-7, cleaved caspase-9 and cleaved PARP, but decreased Bcl-2 expression. In addition, goniothalamin induced apoptosis via p-JNK1/2 and p-p38 upregulation and inhibited cell survival via p-ERK1/2 and p-Akt downregulation. Notably, goniothalamin induced autophagy through upregulation of Atg7, Atg12-Atg5 conjugation and LC3II. The increased p-p38 and p-JNK1/2 and decreased p-Akt may lead to autophagy induction. Therefore, goniothalamin promoted apoptosis associated with autophagy induction in SK-BR-3 cells through p-p38 and p-JNK1/2 upregulation and p-Akt downregulation. The present study indicated that goniothalamin may be further used as a potential therapeutic candidate or may offer an alternative treatment for breast cancer.
Subject(s)
Antineoplastic agent
Caspase 7
Caspase 9
Goniothalamin
Mitogen activated protein kinase
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
Plant medicinal product
Protein Bax
Protein bcl 2
Protein kinase B
Stress activated protein kinase 1
Unclassified drug
Antineoplastic agent
Goniothalamin
Protein kinase B
Pyrone derivative
Antineoplastic activity
Antiproliferative activity
Apoptosis
Article
Autophagy
Cell survival
Controlled study
DNA fragmentation
Down regulation
Human
Human cell
IC50
Priority journal
Protein cleavage
Protein expression
Signal transduction
Upregulation
Apoptosis
Autophagy
Breast Neoplasms
Cell proliferation
Drug effects
Drug screening
Female
Metabolism
Mitochondrial membrane potential
Tumor cell line
Antineoplastic Agents
Apoptosis
Autophagy
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Drug Screening Assays, Antitumor
Female
Humans
MAP Kinase Signaling System
Membrane Potential, Mitochondrial
Proto-Oncogene Proteins c-akt
Pyrones
Caspase 7
Caspase 9
Goniothalamin
Mitogen activated protein kinase
Mitogen activated protein kinase 1
Mitogen activated protein kinase 3
Mitogen activated protein kinase p38
Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
Plant medicinal product
Protein Bax
Protein bcl 2
Protein kinase B
Stress activated protein kinase 1
Unclassified drug
Antineoplastic agent
Goniothalamin
Protein kinase B
Pyrone derivative
Antineoplastic activity
Antiproliferative activity
Apoptosis
Article
Autophagy
Cell survival
Controlled study
DNA fragmentation
Down regulation
Human
Human cell
IC50
Priority journal
Protein cleavage
Protein expression
Signal transduction
Upregulation
Apoptosis
Autophagy
Breast Neoplasms
Cell proliferation
Drug effects
Drug screening
Female
Metabolism
Mitochondrial membrane potential
Tumor cell line
Antineoplastic Agents
Apoptosis
Autophagy
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Drug Screening Assays, Antitumor
Female
Humans
MAP Kinase Signaling System
Membrane Potential, Mitochondrial
Proto-Oncogene Proteins c-akt
Pyrones