Publication: Differential Influence of Imidazolium Ionic Liquid on Cellulase Kinetics in Saccharification of Cellulose and Lignocellulosic Biomass Substrate
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Issued Date
2022
Resource Type
Language
eng
File Type
application/pdf
ISSN
26729156
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
International Journal of Information and Education Technology. Vol 12, No.4 (2022), p.313-317
Suggested Citation
Tantayotai P., Gundupalli M.P., Panakkal E.J., Sriariyanun M., Rattanaporn K., Bhattacharyya D. Differential Influence of Imidazolium Ionic Liquid on Cellulase Kinetics in Saccharification of Cellulose and Lignocellulosic Biomass Substrate. International Journal of Information and Education Technology. Vol 12, No.4 (2022), p.313-317. doi:10.14416/j.asep.2021.11.003 Retrieved from: https://hdl.handle.net/20.500.14740/9130
Abstract
The effect of [Emim][OAc] on Celluclast 1.5 L, Accellerase 1500, and IL-tolerant (MSL2) cellulase during the saccharification of carboxymethylcellulose (CMC), Avicel (AV), rice straw (RS) was studied in one pot process (pretreatment and saccharification). The inhibition caused by [Emim][OAc] (0.5–2.0 M) with substrate loading (20–50 mg/mL) were also evaluated. In most cases, the inhibition mode of saccharification for CMC and AV was identified to be uncompetitive inhibition when the concentration of [Emim][OAc] was higher than 0.5 M. Under the influence of 0.5 M of [Emim][OAc], the Critical Concentration of Substrate (CCS) values of the Celluclast 1.5 L and Accellerase 1500 on CMC hydrolysis were determined to be at 26.59 and 33.65 mg/mL, respectively. Also, increasing in [Emim][OAc] concentration could increase in CCS values, suggesting the positive effect of [Emim][OAc] on the improvement of enzymatic saccharification. This study provides insight into the process optimization for integration of [Emim][OAc] in one pot process of biorefinery. © 2022 King Mongkut’s University of Technology North Bangkok. All Rights Reserved.