Publication:
N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

1040452X

DOI

10.1002/mrd.23122

Other identifier(s)

2-s2.0-85061480593

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular Reproduction and Development. Vol 86, No.4 (2019), p.440-449

Suggested Citation

Timklay W., Magerd S., Sato C., Somrit M., Watthammawut A., Senarai T., Weerachatyanukul W., Kitajima K., Asuvapongpatana S. N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction. Molecular Reproduction and Development. Vol 86, No.4 (2019), p.440-449. doi:10.1002/mrd.23122 Retrieved from: https://hdl.handle.net/20.500.14740/5400

Title

N-linked mannose glycoconjugates on shrimp thrombospondin, pmTSP-II, and their involvement in the sperm acrosome reaction

Author(s)

Timklay W.
 Magerd S.
 Sato C.
 Somrit M.
 Watthammawut A.
 Senarai T.
 Weerachatyanukul W.
 Kitajima K.
 Asuvapongpatana S.

Abstract

Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes. Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin (pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon. A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains. The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor). The function of the Man glycoconjugates was evident by the Con A interference with the pmTSP-II-induced acrosome reaction (AR) as well as by the ability to recover the induction of the AR by the inclusion of Mans in the treatment mixture. In addition, the recombinant proteins of the three signature pmTSP-II domains expressed in E. coli (lacking glycosylation) and mannosidase-treated pmTSP-II showed a minimal ability to initiate the AR response. Together, these results provide evidence of the pivotal role that Man-linked pmTSP-II plays in modulating the shrimp sperm AR, a novel role for a TSP family protein in shrimp reproductive biology. © 2019 Wiley Periodicals, Inc.

Subject(s)

Concanavalin A
 Glycoconjugate
 Lectin
 Mannose
 Mannosidase
 Recombinant protein
 Thrombospondin 2
 Arthropod protein
 Glycoconjugate
 Thrombospondin
 Acrosome reaction
 Article
 Glycosylation
 Histochemistry
 Human
 Immunolocalization
 Native polyacrylamide gel electrophoresis
 Nonhuman
 Penaeus monodon
 Physiology
 Priority journal
 Protein function
 Sperm
 Western blotting
 Animal
 Cytology
 Female
 Genetics
 Male
 Metabolism
 Penaeidae
 Spermatozoon
 Acrosome Reaction
 Animals
 Arthropod Proteins
 Female
 Glycoconjugates
 Glycosylation
 Male
 Penaeidae
 Spermatozoa
 Thrombospondins

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URI

https://hdl.handle.net/20.500.14740/5400

Collections

Scopus: Year 1983-2021

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