Publication:
Is species identification of Echinostoma revolutum using mitochondrial DNA barcoding feasible with high-resolution melting analysis?

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Issued Date

2019

Resource Type

Article

File Type

application/pdf

ISSN

9320113

DOI

10.1007/s00436-019-06322-w

Other identifier(s)

2-s2.0-85065140271

Rights Holder(s)

Scopus

Bibliographic Citation

Parasitology Research. Vol 118, No.6 (2019), p.1799-1810

Suggested Citation

Buddhachat K., Chontananarth T. Is species identification of Echinostoma revolutum using mitochondrial DNA barcoding feasible with high-resolution melting analysis?. Parasitology Research. Vol 118, No.6 (2019), p.1799-1810. doi:10.1007/s00436-019-06322-w Retrieved from: https://hdl.handle.net/20.500.14740/5319

Title

Is species identification of Echinostoma revolutum using mitochondrial DNA barcoding feasible with high-resolution melting analysis?

Author(s)

Buddhachat K.
 Chontananarth T.

Abstract

The taxonomic evaluation of Echinostoma species is controversial. Echinostoma species are recognized as complex, leading to problems associated with accurate identification of these species. The aim of this study was to test the feasibility of using DNA barcoding of cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit 1 (ND1) conjugated with high-resolution melting (HRM) analysis to identify Echinostoma revolutum. HRM using COI and ND1 was unable to differentiate between species in the “revolutum complex” but did distinguish between two isolates of 37-collar-spined echinostome species, including E. revolutum (Asian lineage) and Echinostoma sp. A from different genera, e.g., Hypoderaeum conoideum, Haplorchoides mehrai, Fasciola gigantica, and Thapariella anastomusa, based on the Tm values derived from HRM analysis. Through phylogenetic analysis, a new clade of the cryptic species known as Echinostoma sp. A was identified. In addition, we found that the E. revolutum clade of ND1 phylogeny obtained from the Thailand strain was from a different lineage than the Eurasian lineage. These findings reveal the complexity of the clade, which is composed of 37-collar-spined echinostome species found in Southeast Asia. Taken together, the systematic aspects of the complex revolutum group are in need of extensive investigation by integrating morphological, biological, and molecular features in order to clarify them, particularly in Southeast Asia. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Subject(s)

Cytochrome c oxidase
 Mitochondrial DNA
 Reduced nicotinamide adenine dinucleotide dehydrogenase
 Mitochondrial DNA
 Article
 Cercaria
 Cladistics
 DNA barcoding
 Echinostoma revolutum
 Echinostomatidae
 Evaluation study
 Fasciola gigantica
 Gene locus
 Haplorchoides mechrai
 High resolution melting analysis
 Hypoderaeum conoideum
 Metacercaria
 Nonhuman
 Phylogeny
 Priority journal
 Southeast Asia
 Species identification
 Thailand
 Thapariella anastomusa
 Animal
 Chemistry
 Classification
 DNA barcoding
 Echinostoma
 Genetics
 Isolation and purification
 Mitochondrion
 Transition temperature
 Animals
 Asia, Southeastern
 DNA Barcoding, Taxonomic
 DNA, Mitochondrial
 Echinostoma
 Mitochondria
 Phylogeny
 Thailand
 Transition Temperature

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URI

https://hdl.handle.net/20.500.14740/5319

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Scopus: Year 1983-2021

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