Publication:
Protective effects of γ-mangostin on 6-OHDA-induced toxicity in SH-SY5Y cells

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

3043940

DOI

10.1016/j.neulet.2017.11.059

Other identifier(s)

2-s2.0-85037674221

Rights Holder(s)

Scopus

Bibliographic Citation

Neuroscience Letters. Vol 665, (2018), p.229-235

Suggested Citation

Jaisin Y., Ratanachamnong P., Kuanpradit C., Khumpum W., Suksamrarn S. Protective effects of γ-mangostin on 6-OHDA-induced toxicity in SH-SY5Y cells. Neuroscience Letters. Vol 665, (2018), p.229-235. doi:10.1016/j.neulet.2017.11.059 Retrieved from: https://hdl.handle.net/20.500.14740/3704

Title

Protective effects of γ-mangostin on 6-OHDA-induced toxicity in SH-SY5Y cells

Author(s)

Jaisin Y.
 Ratanachamnong P.
 Kuanpradit C.
 Khumpum W.
 Suksamrarn S.

Abstract

γ-Mangostin is a xanthone with hydroxyl groups that confer the substance-free radical scavenging effects. As opposed to the other more extensively studied mangostins, scarce research has been conducted on neuroprotective effects of γ-mangostin on models of Parkinson's disease (PD). Therefore, this investigation aimed to elucidate its antioxidant and neuroprotective effects on 6-OHDA-induced toxicity in SH-SY5Y cells. 6-OHDA treatment, an inducer of PD pathology in vitro studies, decreased cell viability and increased the level of intracellular ROS production. Furthermore, the substance-induced the expression of phosphorylated p38 MAPK, negatively affected the Bax/Bcl-2 ratio and increased caspase-3 activity; all of which were factors that are associated with apoptosis. Pretreatment of cells with γ-mangostin at concentrations of 0.5, 1, and 2.5 μM markedly increased cell survival and reduced the level of intracellular ROS formation as shown by DPPH radical scavenging activity of the compound. Furthermore, a significant suppression of p-p38, improved Bax/Bcl-2 ratio expression, and reduced caspase-3 activity was exhibited in the cells after γ-mangostin pretreatment. The reduction of apoptosis was further supported by the reduction of pyknotic nuclei indicated by Hoescht 33342 staining. These findings indicate that γ-mangostin could attenuate 6-OHDA-induced neuronal cell death and that the protective effect of γ-mangostin is associated with its antioxidative potential and through the modulation of the apoptotic signalling pathway. Therefore, γ-mangostin may be an effective xanthone among other mangostins for preventing neurodegeneration in PD caused by oxidative stress. © 2017 Elsevier B.V.

Subject(s)

Antioxidant
 Caspase 3
 Gamma mangostin
 Hoe 33342
 Mitogen activated protein kinase 14
 Neuroprotective agent
 Oxidopamine
 Protein Bax
 Protein bcl 2
 Reactive oxygen metabolite
 Synaptophysin
 Trolox C
 Unclassified drug
 Xanthone derivative
 Mangostin
 Neuroprotective agent
 Oxidopamine
 Reactive oxygen metabolite
 Xanthone derivative
 Antioxidant activity
 Apoptosis
 Article
 Cell death
 Cell survival
 Cell viability
 Controlled study
 Cytotoxicity
 DPPH radical scavenging assay
 Drug efficacy
 Enzyme activity
 Human
 Human cell
 IC50
 In vitro study
 Neuroprotection
 Neurotoxicity
 Parkinson disease
 Priority journal
 Protein expression
 SH-SY5Y cell line
 Signal transduction
 Upregulation
 Drug effect
 Metabolism
 Nerve cell
 Tumor cell line
 Apoptosis
 Cell Death
 Cell Line, Tumor
 Cell Survival
 Humans
 Neurons
 Neuroprotective Agents
 Oxidopamine
 Reactive Oxygen Species
 Xanthones

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URI

https://hdl.handle.net/20.500.14740/3704

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Scopus: Year 1983-2021

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