Publication: Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
23793694
Other identifier(s)
2-s2.0-85118974642
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
ACS Sensors. Vol 6, No.11 (2021), p.4067-4075
Suggested Citation
Samper I.C., Sánchez-Cano A., Khamcharoen W., Jang I., Siangproh W., Baldrich E., Geiss B.J., Dandy D.S., Henry C.S. Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care. ACS Sensors. Vol 6, No.11 (2021), p.4067-4075. doi:10.1021/acssensors.1c01527 Retrieved from: https://hdl.handle.net/20.500.14740/8045
Abstract
Rapid and inexpensive serological tests for SARS-CoV-2 antibodies are needed to conduct population-level seroprevalence surveillance studies and can improve diagnostic reliability when used in combination with viral tests. Here, we report a novel low-cost electrochemical capillary-flow device to quantify IgG antibodies targeting SARS-CoV-2 nucleocapsid proteins (anti-N antibody) down to 5 ng/mL in low-volume (10 μL) human whole blood samples in under 20 min. No sample preparation is needed as the device integrates a blood-filtration membrane for on-board plasma extraction. The device is made of stacked layers of a hydrophilic polyester and double-sided adhesive films, which create a passive microfluidic circuit that automates the steps of an enzyme-linked immunosorbent assay (ELISA). The sample and reagents are sequentially delivered to a nitrocellulose membrane that is modified with a recombinant SARS-CoV-2 nucleocapsid protein. When present in the sample, anti-N antibodies are captured on the nitrocellulose membrane and detected via chronoamperometry performed on a screen-printed carbon electrode. As a result of this quantitative electrochemical readout, no result interpretation is required, making the device ideal for point-of-care (POC) use by non-trained users. Moreover, we show that the device can be coupled to a near-field communication potentiostat operated from a smartphone, confirming its true POC potential. The novelty of this work resides in the integration of sensitive electrochemical detection with capillary-flow immunoassay, providing accuracy at the point of care. This novel electrochemical capillary-flow device has the potential to aid the diagnosis of infectious diseases at the point of care. ©
Subject(s)
Antibodies
Blood
Blood vessels
Chemical detection
Diagnosis
Diseases
Electrodes
Immunology
Microfiltration
Recombinant proteins
Viruses
Capillary-flow device
Electrochemicals
Flow devices
Flow immunoassay
Nitrocellulose membranes
Nucleocapsid proteins
Point of care
Point of care diagnostic
Population levels
Sar-cov-2
SARS
Nucleocapsid protein
Virus antibody
Human
Immunoassay
Point of care system
Reproducibility
Seroepidemiology
Antibodies, Viral
COVID-19
Humans
Immunoassay
Nucleocapsid Proteins
Point-of-Care Systems
Reproducibility of Results
SARS-CoV-2
Seroepidemiologic Studies
Blood
Blood vessels
Chemical detection
Diagnosis
Diseases
Electrodes
Immunology
Microfiltration
Recombinant proteins
Viruses
Capillary-flow device
Electrochemicals
Flow devices
Flow immunoassay
Nitrocellulose membranes
Nucleocapsid proteins
Point of care
Point of care diagnostic
Population levels
Sar-cov-2
SARS
Nucleocapsid protein
Virus antibody
Human
Immunoassay
Point of care system
Reproducibility
Seroepidemiology
Antibodies, Viral
COVID-19
Humans
Immunoassay
Nucleocapsid Proteins
Point-of-Care Systems
Reproducibility of Results
SARS-CoV-2
Seroepidemiologic Studies