Publication: Antimicrobial, antimalarial and cytoxicity activities of constituents of a Bhutanese variety of Ajania nubigena
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Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
1934578X
Other identifier(s)
2-s2.0-84886406698
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Natural Product Communications. Vol 8, No.6 (2013), p.733-736
Suggested Citation
Wangchuk P., Keller P.A., Pyne S.G., Korth J., Samten, Taweechotipatr M., Rattanajak R., Kamchonwongpaisan S. Antimicrobial, antimalarial and cytoxicity activities of constituents of a Bhutanese variety of Ajania nubigena. Natural Product Communications. Vol 8, No.6 (2013), p.733-736. doi:10.1177/1934578x1300800613 Retrieved from: https://hdl.handle.net/20.500.14740/6648
Abstract
An investigation of the essential oil (EO) and the crude MeOH extract of a Bhutanese variety of Ajania nubigena using GC/GC-MS and NMR found the following: a) one kg of the dried plant material contained 0.7% w/w EO; b) 44 of the 53 GC-FID peaks of the EO were identified with (3R,6R)-linalool oxide acetate (75.8%) as the major constituent (chemotype II) and chamazulene as a new sub-chemotype; c) purification of the EO furnished (3R,6R)-linalool oxide acetate (1), chamazulene (2), (E)-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4] non-3-ene (3), and (Z)-2-(2,4-hexadinylidene)-1,6-dioxaspiro[4,4]non-3-ene (4); d) from the crude MeOH extract, four flavonoid compounds: 1-(4-hydroxyphenyl) propan-1-one (5), oxyanin B (6), luteolin (7) (major) and the luteolin-7-O-β-D-glucoside (8) were isolated; e) among the EO and pure compounds tested for biological activities, compound 7 exhibited a broad range of moderate antiplasmodial, cytoxicity and antimicrobial activities; c) compound 8 showed significant in vitro antiplasmodial activity against P. falciparum strains TM4/8.2 and K1CB1 (multidrug resistant strain) and was identified as a potential antimalarial scaffold; and f) the in vitro antimicrobial and cytotoxicity activities were in alignment with the traditional medical uses of this plant and thus substantiate its use in Bhutanese traditional medicine.