Publication: Immunohistochemical localization of cathepsin L and cystatin a in normal skin and skin tumors
0
0
Issued Date
2002
Resource Type
File Type
application/pdf
ISSN
3852407
Other identifier(s)
2-s2.0-0036746693
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Dermatology. Vol 29, No.9 (2002), p.573-579
Suggested Citation
Palungwachira P., Kakuta M., Yamazaki M., Yaguchi H., Tsuboi R., Takamori K., Ogawa H. Immunohistochemical localization of cathepsin L and cystatin a in normal skin and skin tumors. Journal of Dermatology. Vol 29, No.9 (2002), p.573-579. doi:10.1111/j.1346-8138.2002.tb00182.x Retrieved from: https://hdl.handle.net/20.500.14740/6705
Abstract
Cathepsin L, a cysteine proteinase, and cystatin A, an inhibitor of cysteine proteinases, are thought to regulate the invasion and metastasis of malignant cells. In this study, the expression of cathepsin L and cystatin A in skin tumors was investigated immunohistochemically in order to examine the relationship between these two enzymes in the pathophysiology of malignant cells. Formalin-fixed and paraffin embedded specimens from normal skin, seborrheic keratoses, and squamous cell carcinomas were reacted with polyclonal antibodies against rat cathepsin L or cystatin α which cross-react to human cathepsin L and cystatin A, respectively. The consequent immunostaining of these enzymes was observed to be strong in normal skin (4 cases) and seborrheic keratosis (6 cases). In well-differentiated squamous cell carcinoma (SCC) (9 cases), staining for cathepsin L and cystatin A was moderately positive in differentiated tumor cells and negative in undifferentiated SCC (5 cases). The degree of staining of these enzymes was inversely correlated with the differentiation of the malignant cells. These results suggest that the immunohistochemical analysis of cathepsin L and cystatin A is a useful indicator for an aspect of malignancy in human epidermal keratinocytes.
Subject(s)
Cathepsin L
Cysteine proteinase
Formaldehyde
Paraffin
Polyclonal antibody
Stefin A
Article
Cancer invasion
Clinical article
Controlled study
Human
Human cell
Human tissue
Immunohistochemistry
Keratinocyte
Metastasis
Protein expression
Protein localization
Seborrheic keratosis
Skin tumor
Squamous cell carcinoma
Cysteine proteinase
Formaldehyde
Paraffin
Polyclonal antibody
Stefin A
Article
Cancer invasion
Clinical article
Controlled study
Human
Human cell
Human tissue
Immunohistochemistry
Keratinocyte
Metastasis
Protein expression
Protein localization
Seborrheic keratosis
Skin tumor
Squamous cell carcinoma