Publication:
Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens

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Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

10759964

DOI

10.1016/j.anaerobe.2008.02.001

Other identifier(s)

2-s2.0-44649149490

Rights Holder(s)

Scopus

Bibliographic Citation

Anaerobe. Vol 14, No.3 (2008), p.166-171

Suggested Citation

Spinler J.K., Taweechotipatr M., Rognerud C.L., Ou C.N., Tumwasorn S., Versalovic J. Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. Vol 14, No.3 (2008), p.166-171. doi:10.1016/j.anaerobe.2008.02.001 Retrieved from: https://hdl.handle.net/20.500.14740/4017

Title

Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens

Author(s)

Spinler J.K.
 Taweechotipatr M.
 Rognerud C.L.
 Ou C.N.
 Tumwasorn S.
 Versalovic J.

Abstract

Lactobacillus reuteri is a commensal-derived anaerobic probiotic that resides in the human gastrointestinal tract. L. reuteri converts glycerol into a potent broad-spectrum antimicrobial compound, reuterin, which inhibits the growth of gram-positive and gram-negative bacteria. In this study, we compared four human-derived L. reuteri isolates (ATCC 55730, ATCC PTA 6475, ATCC PTA 4659 and ATCC PTA 5289) in their ability to produce reuterin and to inhibit the growth of different enteric pathogens in vitro. Reuterin was produced by each of the four L. reuteri strains and assessed for biological activity. The minimum inhibitory concentration (MIC) of reuterin derived from each strain was determined for the following enteric pathogens: enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, Salmonella enterica, Shigella sonnei and Vibrio cholerae. We also analyzed the relative abilities of L. reuteri to inhibit enteric pathogens in a pathogen overlay assay. The magnitude of reuterin production did not directly correlate with the relative ability of L. reuteri to suppress the proliferation of enteric pathogens. Additional antimicrobial factors may be produced by L. reuteri, and multiple factors may act synergistically with reuterin to inhibit enteric pathogens. © 2008 Elsevier Ltd. All rights reserved.

Subject(s)

3 hydroxypropionaldehyde
 Antiinfective agent
 Glycerol
 Glycerol derivative
 Probiotic agent
 Reuterin
 Absorption spectrophotometry
 Article
 Bacterial growth
 Bacterium culture
 Enterohemorrhagic Escherichia coli
 Enterotoxigenic Escherichia coli
 Gastroenteritis
 Gram negative bacterium
 Gram positive bacterium
 Growth inhibition
 High performance liquid chromatography
 Intestine flora
 Lactic acid bacterium
 Lactobacillus
 Lactobacillus reuteri
 Minimum inhibitory concentration
 Nonhuman
 Priority journal
 Salmonella enterica
 Shigella sonnei
 Strain difference
 Vibrio cholerae
 Antibiosis
 Enterohemorrhagic Escherichia coli
 Enterotoxigenic Escherichia coli
 Female
 Gastroenteritis
 Glyceraldehyde
 Gram-Negative Bacteria
 Humans
 Intestines
 Lactobacillus reuteri
 Microbial Sensitivity Tests
 Probiotics
 Propane
 Salmonella enterica
 Shigella sonnei
 Vibrio cholerae
 Bacteria (microorganisms)
 Escherichia coli
 Lactobacillus reuteri
 Negibacteria
 Posibacteria
 Salmonella enterica
 Shigella sonnei
 Vibrio cholerae

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URI

https://hdl.handle.net/20.500.14740/4017

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Scopus: Year 1983-2021

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