Publication:
Direct injection of human serum and pharmaceutical formulations for glucosamine determination by CE-C 4D method

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

15700232

DOI

10.1016/j.jchromb.2011.05.045

Other identifier(s)

2-s2.0-79960560178

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol 879, No.23 (2011), p.2185-2188

Suggested Citation

Chaisuwan P., Kongprasertsak T., Sangcakul A., Smith N.W., Nachapricha D., Wilairat P., Uraisin K. Direct injection of human serum and pharmaceutical formulations for glucosamine determination by CE-C 4D method. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol 879, No.23 (2011), p.2185-2188. doi:10.1016/j.jchromb.2011.05.045 Retrieved from: https://hdl.handle.net/20.500.14740/7274

Title

Direct injection of human serum and pharmaceutical formulations for glucosamine determination by CE-C 4D method

Author(s)

Chaisuwan P.
 Kongprasertsak T.
 Sangcakul A.
 Smith N.W.
 Nachapricha D.
 Wilairat P.
 Uraisin K.

Abstract

A simple CE-C 4D method has been developed for the determination of glucosamine by direct injection of human serum and pharmaceutical samples. Glucosamine was electrokinetically injected and analysed in its protonated form using 20mM MES/His (pH 6) as background electrolyte in order to separate it from the matrix and to provide a better response to the C 4D detector. Separation of glucosamine in human serum and pharmaceutical samples was performed in 3min without the need for protein precipitation or matrix removal. Good precision in terms of %RSD for the migration time and peak area were less than 1.91% (n=10). The conductivity signal was linear with glucosamine concentration in the range 0.10-2.50mg/mL, with a detection limit of 0.03mg/mL. Recoveries of glucosamine in serum and pharmaceutical samples were 86.5-104.78%. The method was successfully applied for the determination of the glucosamine content in pharmaceutical formulations and validated with high performance liquid chromatography (HPLC). Good agreements were observed between the developed method, label values and the HPLC method. Glucosamine could be detected in spiked serum sample by direct injection. This was not possible by HPLC due to co-eluting interferences. © 2011 Elsevier B.V.

Subject(s)

Back ground electrolyte
 Capacitively coupled contactless conductivity detection
 Detection limits
 HPLC method
 Human serum
 Matrix
 Matrix removal
 Migration time
 Peak area
 Pharmaceutical formulation
 Protein precipitation
 Protonated
 Serum samples
 Body fluids
 Capillary electrophoresis
 Chromatography
 Detectors
 Glucosamine
 High performance liquid chromatography
 Precipitation (chemical)
 Direct injection
 Electrolyte
 Glucosamine
 Article
 Capillary electrophoresis
 Capillary electrophoresis capacitively coupled contactless conductivity detection
 Carbohydrate analysis
 Conductance
 Drug formulation
 High performance liquid chromatography
 Priority journal
 Proton transport
 Serum
 Chemistry, Pharmaceutical
 Electrophoresis, Capillary
 Glucosamine
 Humans
 Pharmaceutical Preparations
 Serum

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URI

https://hdl.handle.net/20.500.14740/7274

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Scopus: Year 1983-2021

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