Publication: Growth inhibition of cholic acid-conjugated polyethylenimine 25K/ p53-EGFP complexes on human carcinoma cells
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
22313354
Other identifier(s)
2-s2.0-85104009065
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Applied Pharmaceutical Science. Vol 11, No.4 (2021), p.14-21
Suggested Citation
Weecharangsan W., Niyomtham N., Yingyongnarongkul B.E., Opanasopit P., Lee R.J. Growth inhibition of cholic acid-conjugated polyethylenimine 25K/ p53-EGFP complexes on human carcinoma cells. Journal of Applied Pharmaceutical Science. Vol 11, No.4 (2021), p.14-21. doi:10.7324/JAPS.2021.110403 Retrieved from: https://hdl.handle.net/20.500.14740/7843
Abstract
Cholic acid-conjugated polyethylenimine 25K (PEI 25K-CA) was synthesized and characterized for gene delivery of its buffer capacity, PEI 25K-CA/plasmid DNA complex, zeta potential and particle size, transfection efficiency, cytotoxicity, and carcinoma cell growth inhibition. PEI 25K-CA was complexed with plasmid DNA at the ratio above 0.25/1. PEI 25K-CA/p53-EGFP (p53-enhanced green fluorescent protein) and PEI 25K/p53-EGFP had the particle sizes of 238.2 ± 3.1–529.1 ± 3.6 nm, and the zeta potential of 3.7 ± 1.6–4.3 ± 0.2 mV, respectively. The particle size of the complexes increased with the period of time. PEI 25K-CA/p53-EGFP had transfection efficiency as PEI 25K/ p53-EGFP. The cytotoxicity of PEI 25K-CA was dependent on the concentration of the polymer. The growth inhibition activity was dependent on the dose of p53-EGFP. PEI 25K-CA/pEGFP had better colloidal stability than PEI 25K-CA/ pEGFP. This study concluded that PEI 25K-CA had efficient gene delivery activity as PEI and had lower cytotoxicity and more colloidal stability than PEI 25K. © 2021 Wanlop Weecharangsan et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).