Publication: Development of biodegradation process for Poly(DL-lactic acid)degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1
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0
Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
7173458
Other identifier(s)
2-s2.0-85065887289
Rights Holder(s)
Scopus
Bibliographic Citation
Electronic Journal of Biotechnology. Vol 40, (2019), p.52-57
Suggested Citation
Panyachanakul T., Sorachart B., Lumyong S., Lorliam W., Kitpreechavanich V., Krajangsang S. Development of biodegradation process for Poly(DL-lactic acid)degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1. Electronic Journal of Biotechnology. Vol 40, (2019), p.52-57. doi:10.1016/j.ejbt.2019.04.005 Retrieved from: https://hdl.handle.net/20.500.14740/5272
Abstract
Background: Plastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid)(PLA)is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This study focused on the scale-up and evaluated the bioprocess of PLA degradation by a crude microbial enzyme produced by Actinomadura keratinilytica strain T16-1 in a 5 L stirred tank bioreactor. Results: PLA degradation after 72 h in a 5 L bioreactor by using the enzyme of the strain T16-1 under controlled pH conditions resulted in lactic acid titers (mg/L)of 16,651 mg/L and a conversion efficiency of 89% at a controlled pH of 8.0. However, the PLA degradation process inadvertently produced lactic acid as a potential inhibitor, as shown in our experiments at various concentrations of lactic acid. Therefore, the dialysis method was performed to reduce the concentration of lactic acid. The experiment with a dialysis bag achieved PLA degradation by weight loss of 99.93%, whereas the one without dialysis achieved a degradation of less than approximately 14.75%. Therefore, the dialysis method was applied to degrade a commercial PLA material (tray)with a conversion efficiency of 32%, which was 6-fold more than that without dialysis. Conclusions: This is the first report demonstrating the scale-up of PLA degradation in a 5 L bioreactor and evaluating a potential method for enhancing PLA degradation efficiency. How to cite: Panyachanakul T, Sorachart B, Lumyong S, et al. Development of biodegradation process for Poly(DL-lactic acid)degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1. Electron J Biotechnol 2019;40. https://doi.org/10.1016/j.ejbt.2019.04.005 © 2019
Subject(s)
Biodegradation
Biological water treatment
Bioreactors
Conversion efficiency
Degradation
Dialysis
Efficiency
Enzymes
Scales (weighing instruments)
Wastewater
Actinomadura keratinilytica
Degrading enzymes
Dialysis method
Plastic wastes
Scale-up
Weight loss
Lactic acid
Bacterial enzyme
Polylactic acid
Actinomadura
Actinomadura keratinilytica
Article
Bacterial strain
Biodegradation
Concentration (parameter)
Controlled study
Dialysis
Nonhuman
PH
Pore size
Scale up
Biological water treatment
Bioreactors
Conversion efficiency
Degradation
Dialysis
Efficiency
Enzymes
Scales (weighing instruments)
Wastewater
Actinomadura keratinilytica
Degrading enzymes
Dialysis method
Plastic wastes
Scale-up
Weight loss
Lactic acid
Bacterial enzyme
Polylactic acid
Actinomadura
Actinomadura keratinilytica
Article
Bacterial strain
Biodegradation
Concentration (parameter)
Controlled study
Dialysis
Nonhuman
PH
Pore size
Scale up