Publication: Potential use of amla (Phyllanthus emblica l.) fruit extract to protect skin keratinocytes from inflammation and apoptosis after uvb irradiation
0
0
Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
20763921
Other identifier(s)
2-s2.0-85113278254
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Antioxidants. Vol 10, No.5 (2021)
Suggested Citation
Kunchana K., Jarisarapurin W., Chularojmontri L., Wattanapitayakul S.K. Potential use of amla (Phyllanthus emblica l.) fruit extract to protect skin keratinocytes from inflammation and apoptosis after uvb irradiation. Antioxidants. Vol 10, No.5 (2021). doi:10.3390/antiox10050703 Retrieved from: https://hdl.handle.net/20.500.14740/7806
Abstract
Ultraviolet B (UVB) exposure is the primary risk factor for the deadliest type of skin cancer—melanoma. Incorporating natural antioxidants in skin protection products is currently a favored research theme. For this study, we selected Phyllanthus emblica L. fruit extract (PE) to assess its potential use in dermal protection against UVB-induced keratinocyte inflammation and apoptosis. High-performance liquid chromatography (HPLC) was used to investigate PE’s phyto-chemical constituents (ascorbic acid, ellagic acid, gallic acid, chlorogenic acid, and quercetin), while ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total ROS, OH•, O2•−, and H2O2-scavenging activities were used to determine the antioxidant properties. PE significantly increased the cell viability (MTT assay) and reduced apoptosis (Hoechst staining) in HaCaT cells exposed to UVB (40 mJ/cm2 ). PE abolished oxidative stress by reducing the production of intracellular ROS, O2•− and H2O2 production. Catalase activity (but not superoxide dismutase or glutathione peroxidase activity) was enhanced in keratinocytes incubated with PE prior to UVB exposure. Western blot analysis suggested that PE inhibited cytochrome c release and inhibited the dysregulation of PI3K/Akt without any impact on p38 activation. PE attenuated the inflammatory response to UVB irradiation by inhibiting AP-1, NF-κB, and the mediator PGE2 . Thus, PE is a candidate with great potential for use as an active ingredient in skin care products. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.