Publication: Melatonin protects SK-N-SH neuroblastoma cells from amphetamine-induced neurotoxicity
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0
Issued Date
2007
Resource Type
File Type
application/pdf
ISSN
7423098
Other identifier(s)
2-s2.0-34447118322
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Pineal Research. Vol 43, No.1 (2007), p.65-73
Suggested Citation
Klongpanichapak S., Phansuwan-Pujito P., Ebadi M., Govitrapong P. Melatonin protects SK-N-SH neuroblastoma cells from amphetamine-induced neurotoxicity. Journal of Pineal Research. Vol 43, No.1 (2007), p.65-73. doi:10.1111/j.1600-079X.2007.00444.x Retrieved from: https://hdl.handle.net/20.500.14740/4455
Abstract
Several hypotheses regarding the mechanism underlying amphetamine-induced neurotoxicity have been proposed. One of them is based on the observation of free radical formation and oxidative stress produced by auto-oxidation of dopamine (DA). The formation of DA-related reactive oxygen species (ROS) such as superoxide and hydroxyl radicals appears to play an important role in amphetamine-induced neurotoxicity. Melatonin, the main secretory product of pineal gland, is well known for its protective effects that are currently attributed mainly to its radical scavenging and antioxidant properties. The present study was conducted to investigate the protective effects of melatonin on d-amphetamine (AMPH)-induced neurotoxicity in cultured human dopaminergic neuroblastoma SK-N-SH cells. Our data indicate that AMPH significantly reduces cell viability, induces oxidative stress (enhances ROS production and malondialdehyde levels), up-regulates α-synuclein expression and decreases intracellular ATP levels. However, pretreatment of SK-N-SH cells with melatonin prevents AMPH-induced loss of cell viability and induction of oxidative stress, while reducing α-synuclein expression and increasing ATP production. These results suggest that the antioxidant properties of melatonin may provide a protective mechanism against AMPH-induced neuronal degeneration. © 2007 The Authors.
Subject(s)
Adenosine triphosphate
Alpha synuclein
Amphetamine
Malonaldehyde
Melatonin
Reactive oxygen metabolite
Antioxidant activity
Article
Cell viability
Controlled study
Dopaminergic nerve cell
Human
Human cell
Intracellular space
Neuroblastoma cell
Neuroprotection
Neurotoxicity
Oxidative stress
Protein expression
Upregulation
Cell Line, Tumor
Dextroamphetamine
Humans
Melatonin
Neuroblastoma
Neurons
Alpha synuclein
Amphetamine
Malonaldehyde
Melatonin
Reactive oxygen metabolite
Antioxidant activity
Article
Cell viability
Controlled study
Dopaminergic nerve cell
Human
Human cell
Intracellular space
Neuroblastoma cell
Neuroprotection
Neurotoxicity
Oxidative stress
Protein expression
Upregulation
Cell Line, Tumor
Dextroamphetamine
Humans
Melatonin
Neuroblastoma
Neurons