Publication:
Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

1407775

DOI

10.1111/jfd.13115

Other identifier(s)

2-s2.0-85075465159

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Fish Diseases. Vol 43, No.2 (2020), p.207-214

Suggested Citation

Wangman P., Chaivisuthangkura P., Taengchaiyaphum S., Pengsuk C., Sithigorngul P., Longyant S. Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease. Journal of Fish Diseases. Vol 43, No.2 (2020), p.207-214. doi:10.1111/jfd.13115 Retrieved from: https://hdl.handle.net/20.500.14740/4655

Title

Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease

Author(s)

Wangman P.
 Chaivisuthangkura P.
 Taengchaiyaphum S.
 Pengsuk C.
 Sithigorngul P.
 Longyant S.

Abstract

Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VPAHPND), were employed to develop a rapid strip test. One MAb was conjugated to colloidal gold to bind to ToxB at the application pad, and another MAb was used to capture colloidal gold MAb–protein complexes at the test line (T) on the nitrocellulose strip. To validate test performance, a downstream control line (C) of goat anti-mouse immunoglobulin G antibody was used to capture the free colloidal gold conjugate MAb. The sample in the application buffer could be applied directly to the application well, and the test result was obtained within 15 min. The sensitivity of the kit is approximately 6.25 µg/ml of toxin, which was equivalent to the toxin produced by approximately 107 cfu/ml of bacteria. This kit is convenient and easy to use since it can be used to identify VPAHPND directly using a single colony of bacteria grown on agar culture plates. Because of its high specificity and simplicity, as well as not being reliant on sophisticated equipment or specialized skills, this strip test could be used by farmers for surveillance for ToxB-producing bacteria. © 2019 John Wiley & Sons Ltd

Subject(s)

Agar
 Colloidal gold
 Immunoglobulin G antibody
 Monoclonal antibody
 Phosphate buffered saline
 Pyroxylin
 Toxin
 Toxin b
 Unclassified drug
 Bacterial toxin
 Bacterium antibody
 Acute hepatopancreatic necrosis disease
 Agricultural worker
 Article
 Bacterial growth
 Bacterium colony
 Bacterium culture
 Controlled study
 Immunoaffinity chromatography
 Immunochromatographic strip test
 Liver necrosis
 Nonhuman
 Pancreas necrosis
 Sensitivity and specificity
 Task performance
 Vibrio parahaemolyticus
 Affinity chromatography
 Animal
 Hepatopancreas
 Immunoassay
 Isolation and purification
 Microbiology
 Penaeidae
 Procedures
 Veterinary medicine
 Vibrio parahaemolyticus
 Animals
 Antibodies, Bacterial
 Antibodies, Monoclonal
 Bacterial Toxins
 Chromatography, Affinity
 Hepatopancreas
 Immunoassay
 Penaeidae
 Vibrio parahaemolyticus

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URI

https://hdl.handle.net/20.500.14740/4655

Collections

Scopus: Year 1983-2021

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