Publication: Application of Analytical Methods for the Determination of Bioactive Compounds in Some Berries
0
0
Issued Date
2013
Resource Type
File Type
application/pdf
ISSN
19369751
Other identifier(s)
2-s2.0-84875053039
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Food Analytical Methods. Vol 6, No.2 (2013), p.432-444
Suggested Citation
Gorinstein S., Arancibia-Avila P., Toledo F., Namiesnik J., Leontowicz H., Leontowicz M., Ham K.-S., Kang S.-G., Vearasilp K., Suhaj M. Application of Analytical Methods for the Determination of Bioactive Compounds in Some Berries. Food Analytical Methods. Vol 6, No.2 (2013), p.432-444. doi:10.1007/s12161-012-9453-z Retrieved from: https://hdl.handle.net/20.500.14740/6692
Abstract
Fluorometry, ESI-MS, FTIR, and radical scavenging assays were used for characterization of bioactive compounds and the levels of their antioxidant activities. Polyphenols, flavonoids, anthocyanins, and ascorbic acid and the level of antioxidant activity of water extracts of "Murtilla-like" [Myrteola nummularia (Poiret) Berg.], and other widely consumed berries were determined and compared. The contents of bioactive compounds and the levels of antioxidant activities in water extracts differed significantly in the investigated samples (P < 0.05). "Murtilla-like" extracts contained polyphenols (mg GAE/g)-19.13 ± 0.9, flavonoids, (mg CE/g)-3.12 ± 0.1, anthocyanins (mg CGE/g)-120.23 ± 5. 4, and ascorbic acid (mg/g)-2.20 ± 0.1; and antioxidant activities (μmolTE/g) by ABTS and CUPRAC assays were 200.55 ± 8.7 and CUPRAC 116.76 ± 5.7, respectively. Chemometrical processing was done on the basis of kinetic data of two variables (concentration and reaction time) by DPPH scavenging reaction. Polyphenol content highly correlated with antioxidant capacity (R2 from 0.96 to 0.83). The quenching properties of berries were studied by the interaction of water polyphenol extracts with a small protein such as BSA by 3-D fluorescence and FTIR spectroscopy. These methods were used as additional tools for the characterization of polyphenols. Wild-grown non-investigated berries were compared with widely consumed ones, using their bioactive composition, antioxidant activities, and antiproliferative and fluorescence properties. In conclusion, the antioxidant properties of "Murtilla-like" can be used as a new source for consumption. The bioactivity of "Murtilla-like" is comparable with blueberries and raspberries. 3-D fluorescence and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of food products. © 2012 Springer Science+Business Media, LLC.
Subject(s)
Anthocyanins
Antioxidants
Bioactivity
Extraction
Fluorescence
Fourier transform infrared spectroscopy
Fruits
Analytical method
Anti-oxidant activities
Antioxidant capacity
Antioxidant properties
Berries
Bioactive compounds
Fluorescence properties
Scavenging reactions
Ascorbic acid
Myrteola
Rubus glaucus
Vaccinium
Antioxidants
Bioactivity
Extraction
Fluorescence
Fourier transform infrared spectroscopy
Fruits
Analytical method
Anti-oxidant activities
Antioxidant capacity
Antioxidant properties
Berries
Bioactive compounds
Fluorescence properties
Scavenging reactions
Ascorbic acid
Myrteola
Rubus glaucus
Vaccinium