Publication:
QCM-based rapid detection of PCR amplification products of Ehrlichia canis

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2017.10.037

Other identifier(s)

2-s2.0-85035109804

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Analytica Chimica Acta. Vol 1001, (2018), p.106-111

Suggested Citation

Bunroddith K., Viseshakul N., Chansiri K., Lieberzeit P. QCM-based rapid detection of PCR amplification products of Ehrlichia canis. Analytica Chimica Acta. Vol 1001, (2018), p.106-111. doi:10.1016/j.aca.2017.10.037 Retrieved from: https://hdl.handle.net/20.500.14740/3697

Title

QCM-based rapid detection of PCR amplification products of Ehrlichia canis

Author(s)

Bunroddith K.
 Viseshakul N.
 Chansiri K.
 Lieberzeit P.

Abstract

Ehrlichia canis is an intracellular parasitic bacterium and arthropod-borne pathogen that receives growing attention, because it leads to increasing morbidity and mortality in animals. It does so by causing canine monocytotropic ehrlichiosis (CME). Infected canines may lack obvious clinical signs and stay in chronic stage. Herein we report a rapid screening method based on PCR assay combined with quartz crystal microbalance (QCM) to design a DNA sensor for detecting E. canis in early stages of infection. The test relies on DNA amplification of target nucleotide sequences via PCR followed by detecting DNA-DNA hybridization using QCM. The approach did not result in any cross-hybridization toward other blood bacteria or parasites in dogs, such as Anaplasma platys, Babesia canis and Trypanosoma spp, but turned out selective for the target species. The limit of detection of QCM was as low as 4.1 × 109 molecules/μl of 289 bp E. canis PCR products corresponding to 22 copy numbers/μl of E. canis. Furthermore, the technique is also simple, does not require complicated equipment and can in principle be reused. © 2017 Elsevier B.V.

Subject(s)

Bacteria
 DNA
 DNA sequences
 Quartz
 Quartz crystal microbalances
 Cross hybridization
 DNA amplification
 DNA biosensors
 DNA-DNA hybridization
 Ehrlichia canis
 Limit of detection
 Nucleotide sequences
 PCR amplification
 Polymerase chain reaction
 Article
 Bacterium detection
 Blood sampling
 Controlled study
 Copy number variation
 Cross hybridization
 DNA hybridization
 Ehrlichia canis
 Gene amplification
 Immobilization
 Limit of detection
 Molecular probe
 Nonhuman
 Nucleotide sequence
 Polymerase chain reaction
 Priority journal
 Process optimization
 Quartz crystal microbalance
 Screening test
 Animal
 Devices
 Dog
 Dog disease
 Ehrlichia canis
 Ehrlichiosis
 Equipment design
 Genetics
 Isolation and purification
 Microbiology
 Polymerase chain reaction
 Procedures
 Quartz crystal microbalance
 Veterinary
 Bacterial DNA
 Animals
 DNA, Bacterial
 Dog Diseases
 Dogs
 Ehrlichia canis
 Ehrlichiosis
 Equipment Design
 Limit of Detection
 Polymerase Chain Reaction
 Quartz Crystal Microbalance Techniques

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URI

https://hdl.handle.net/20.500.14740/3697

Collections

Scopus: Year 1983-2021

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