Publication:
Estrogen down-regulates glial activation in male mice following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

68993

DOI

10.1016/j.brainres.2006.02.029

Other identifier(s)

2-s2.0-33646388397

Rights Holder(s)

Scopus

Bibliographic Citation

Brain Research. Vol 1084, No.1 (2006), p.28-37

Suggested Citation

Tripanichkul W., Sripanichkulchai K., Finkelstein D.I. Estrogen down-regulates glial activation in male mice following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication. Brain Research. Vol 1084, No.1 (2006), p.28-37. doi:10.1016/j.brainres.2006.02.029 Retrieved from: https://hdl.handle.net/20.500.14740/5803

Title

Estrogen down-regulates glial activation in male mice following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication

Author(s)

Tripanichkul W.
 Sripanichkulchai K.
 Finkelstein D.I.

Abstract

Emerging evidence suggests beneficial effect of estrogen for Parkinson's disease (PD), yet the exact mechanisms implicated remain obscured. Activated glia observed in MPTP mouse model and in PD may participate in the cascade of deleterious events that ultimately leads to dopaminergic nigral neuronal death. In vitro studies demonstrate that estrogen can modify the microglial and astroglial expression of inflammatory mediator, such as cytokines and chemokines implicated in neuroinflammation and neurodegeneration. To determine whether estrogen-elicited neuroprotection in PD is mediated through glia, adult male C57Bl/6 mice were treated with 17β-estradiol (E2) for a total of 11 days. Following 5 days of pretreatment with E2, they were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) on the sixth day. The brains were collected on day 11. Immunohistochemistry and quantitative study were used to assess the number of tyrosine hydroxylase-immunoreactive (TH-IR) neurons in the substantia nigra pars compacta (SNpc) and that of activated astrocytes and activated microglia in the SNpc and the striatum. Pretreatment with E2 decreased the loss of TH-IR nigral neurons and diminished the deficit of TH-IR striatal fibers triggered by MPTP. The neuroprotective effect of E2 was coincident with an attenuation of a glial response within the nigra and the striatum. These findings suggest that the neuroprotective effects of E2 evidenced in MPTP mouse model might mediate through an inhibition of reactive glia. However, direct neuroprotective effects of E2 upon TH-IR neurons cannot be excluded. © 2006 Elsevier B.V. All rights reserved.

Subject(s)

1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine
 Estradiol
 Estrogen
 Tyrosine 3 monooxygenase
 Animal cell
 Animal model
 Animal tissue
 Article
 Astrocyte
 Cell activation
 Cell loss
 Controlled study
 Corpus striatum
 Disease model
 Down regulation
 Glia cell
 Immunocompetent cell
 Immunohistochemistry
 Injection
 Male
 Mouse
 Nerve cell
 Nerve fiber
 Neuroprotection
 Nonhuman
 Parkinson disease
 Priority journal
 Quantitative analysis
 Substantia nigra
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
 Analysis of Variance
 Animals
 Basal Ganglia
 Cell Count
 Cell Death
 Disease Models, Animal
 Drug Administration Schedule
 Estrogens
 Gene Expression Regulation
 Glial Fibrillary Acidic Protein
 Immunohistochemistry
 Lectins
 Male
 Mice
 Mice, Inbred C57BL
 Neuroglia
 Neurons
 Neurotoxicity Syndromes
 Tyrosine 3-Monooxygenase
 Animalia

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URI

https://hdl.handle.net/20.500.14740/5803

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Scopus: Year 1983-2021

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