Publication:
Effects of ozone treatment on cell growth and ultrastructural changes in bacteria

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

221260

DOI

10.2323/jgam.48.193

Other identifier(s)

2-s2.0-0036444374

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of General and Applied Microbiology. Vol 48, No.4 (2002), p.193-199

Suggested Citation

Thanomsub B., Anupunpisit V., Chanphetch S., Watcharachaipong T., Poonkhum R., Srisukonth C. Effects of ozone treatment on cell growth and ultrastructural changes in bacteria. Journal of General and Applied Microbiology. Vol 48, No.4 (2002), p.193-199. doi:10.2323/jgam.48.193 Retrieved from: https://hdl.handle.net/20.500.14740/6801

Title

Effects of ozone treatment on cell growth and ultrastructural changes in bacteria

Author(s)

Thanomsub B.
 Anupunpisit V.
 Chanphetch S.
 Watcharachaipong T.
 Poonkhum R.
 Srisukonth C.

Abstract

Ozone appeared to inhibit growth and caused the death of gram negative and gram positive tested bacteria: Escherichia coli, Salmonella sp., Staphylococcus aureus and Bacillus subtilis. Bacterial cultures at 103, 104, 105, 106, and 107 cfu/ml dilution were exposed to 0.167/mg/min/L of ozone at different time intervals (0, 5, 10, 15, 30, 60, 90, 120, and 150 min). Cell viability was observed in all types of tested bacteria at 103, 104, 105 cfu/ml within 30 min after ozone exposure. However, cell inactivation was not significantly observed at concentrations of 106, 107 cfu/ml even after an exposure of 150 min. Ultrastructural changes of treated bacteria showed deformation, rough damage and surface destruction revealed by scanning electron microscopy. Some bacterial cells showed collapsed and shrunken patterns within 60 min and severe rupture and cellular lysis after 90 min of ozone treatment. This study supports the proposed mechanism of the bacteria inactivation by ozone that caused cell membrane destruction and finally lysis reaction. Thus, the precaution of using ozone as a biocide should be used to address appropriate concentrations of bacterial contamination in water.

Subject(s)

Biocide
 Ozone
 Article
 Bacillus subtilis
 Bacterial count
 Bacterial growth
 Bacterial membrane
 Bacteriolysis
 Bacterium contamination
 Bacterium culture
 Cell surface
 Cell ultrastructure
 Cell viability
 Colony forming unit
 Controlled study
 Escherichia coli
 Exposure
 Gram negative bacterium
 Gram positive bacterium
 Membrane rupture
 Nonhuman
 Salmonella
 Scanning electron microscopy
 Staphylococcus aureus
 Time
 Water contamination
 Bacillus subtilis
 Bacillus subtilis
 Bacteria (microorganisms)
 Escherichia coli
 Escherichia coli
 Negibacteria
 Posibacteria
 Salmonella
 Salmonella
 Salmonella sp.
 Staphylococcus
 Staphylococcus aureus
 Staphylococcus aureus

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URI

https://hdl.handle.net/20.500.14740/6801

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Scopus: Year 1983-2021

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