Publication:
Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification

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Issued Date

2009

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2009.08.005

Other identifier(s)

2-s2.0-70349734867

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Virological Methods. Vol 162, (2009), p.188-193

Suggested Citation

Chaivisuthangkura P., Srisuk C., Rukpratanporn S., Longyant S., Sridulyakul P., Sithigorngul P. Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification. Journal of Virological Methods. Vol 162, (2009), p.188-193. doi:10.1016/j.jviromet.2009.08.005 Retrieved from: https://hdl.handle.net/20.500.14740/3690

Title

Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification

Author(s)

Chaivisuthangkura P.
 Srisuk C.
 Rukpratanporn S.
 Longyant S.
 Sridulyakul P.
 Sithigorngul P.

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel, sensitive and rapid method for amplification of nucleic acids under isothermal conditions. In this report, a LAMP method was developed for detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV), known previously as monodon baculovirus (MBV), using a set of six primers designed to specifically recognize the PemoNPV polyhedrin gene. The optimized time and temperature conditions for the LAMP assay were 60 min at 63 °C. The sensitivity of LAMP for PemoNPV detection was approximately 50 viral copies ng-1 genomic DNA (equivalent to 150 viral copies per reaction). Using a DNA template extracted from PemoNPV-infected shrimp by a viral nucleic acid kit, the detection limit of LAMP was 0.7 fg while that of nested PCR was 70 fg; therefore, the LAMP assay was 100 times more sensitive than nested PCR. The LAMP method did not amplify a product using nucleic acid extracted from shrimp infected with other viruses including yellow head virus (YHV), Taura syndrome virus (TSV), white spot syndrome virus (WSSV), Penaeus stylirostris densovirus (PstDNV) known previously as infectious hypodermal and hematopoietic necrosis virus (IHHNV), and Penaeus monodon densovirus (PmDNV) known previously as hepatopancreatic parvovirus (HPV). © 2009 Elsevier B.V. All rights reserved.

Subject(s)

Genomic DNA
 Polyhedrin
 Analytical equipment
 Article
 Densovirus
 DNA extraction
 Infectious hematopoietic necrosis virus
 Loop mediated isothermal amplification
 Nonhuman
 Nucleic acid amplification
 Nucleotide sequence
 Parvovirus
 Penaeus monodon nucleopolyhedrovirus
 Penaeus stylirostris densovirus
 Plasmid
 Polyhedrosis virus
 Polymerase chain reaction
 Priority journal
 Reovirus
 Sensitivity analysis
 Shrimp
 Taura syndrome virus
 Temperature
 Virus detection
 Virus gene
 Virus infection
 White spot syndrome virus
 Yellow head virus
 Animals
 DNA Primers
 Nucleic Acid Amplification Techniques
 Nucleopolyhedrovirus
 Penaeidae
 Polymerase Chain Reaction
 Sensitivity and Specificity
 Species Specificity
 Time Factors
 Viral Structural Proteins
 Decapoda (Crustacea)
 Densovirus
 Hepatopancreatic parvovirus of penaeid shrimp
 Infectious hypodermal and hematopoietic necrosis virus
 Litopenaeus stylirostris
 Monodon baculovirus
 Nucleopolyhedrovirus
 Penaeus monodon
 Shrimp white spot syndrome virus
 Taura syndrome virus
 Yellow head virus

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URI

https://hdl.handle.net/20.500.14740/3690

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Scopus: Year 1983-2021

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