Publication:
Streptomyces musisoli sp. nov., an actinomycete isolated from soil

2
0

Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

14665026

DOI

10.1099/ijsem.0.004857

Other identifier(s)

2-s2.0-85108965899

Rights Holder(s)

Scopus

Bibliographic Citation

International Journal of Systematic and Evolutionary Microbiology. Vol 71, No.7 (2021)

Suggested Citation

Duangupama T., Intaraudom C., Pittayakhajonwut P., Suriyachadkun C., Tadtong S., Sirirote P., Tanasupawat S., Thawai C. Streptomyces musisoli sp. nov., an actinomycete isolated from soil. International Journal of Systematic and Evolutionary Microbiology. Vol 71, No.7 (2021). doi:10.1099/ijsem.0.004857 Retrieved from: https://hdl.handle.net/20.500.14740/8025

Title

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

Author(s)

Duangupama T.
 Intaraudom C.
 Pittayakhajonwut P.
 Suriyachadkun C.
 Tadtong S.
 Sirirote P.
 Tanasupawat S.
 Thawai C.

Abstract

An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15:0, iso-C16:0, and anteiso-C17:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanola-mine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells. A combination of morphological and chemotaxonomic data supported the assignment to the genus Strep-tomyces. The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain. The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9%), followed by Streptomyces actinomycinicus RCU-197T (98.9%). The average nucleotide identity by blast (ANIb) and digital DNA–DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6% and ≤47.4%, respectively. The digital DNA G+C content of genomic DNA was 72.1 mol%. On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp. nov. is proposed. The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T). © 2021 The Authors.

Subject(s)

Acetylglucosaminidase
 Alkaline phosphatase
 Alpha galactosidase
 Alpha glucosidase
 Alpha levo fucosidase
 Arabinose
 Aryl acylamidase
 Beta galactosidase
 Beta glucosidase
 Beta glucuronidase
 Cardiolipin
 Cellobiose
 Cellulose
 Chymotrypsin
 Chymotrypsin trypsin
 Cysteine
 Cystine
 Dextran
 Diaminopimelic acid
 Enzyme
 Farnoquinone
 Fatty acid
 Fructose
 Galactose
 Galactosidase
 Genomic DNA
 Glucose
 Histidine
 Hydroxyproline
 Hypoxanthine
 Inositol
 Inulin
 Lactose
 Mannitol
 Mannose
 Mannoside
 Melibiose
 Menaquinone
 Methionine
 N acetyl beta glucosaminidase
 Nitrogen
 Nucleotide
 Peptidoglycan
 Phenylalanine
 Phosphatase
 Phosphatidylethanolamine
 Phosphatidylglycerol
 Phosphatidylinositol
 Phospholipid
 Proline
 Raffinose
 Rhamnose
 Ribitol
 RNA 16S
 Salicin
 Sucrose
 Xanthine
 Xylose
 Bacterial DNA
 Diaminopimelic acid
 Farnoquinone
 Fatty acid
 Menaquinone
 Peptidoglycan
 Phospholipid
 RNA 16S
 Article
 Bacterium isolation
 Chemotaxonomy
 Controlled study
 DNA base composition
 DNA DNA hybridization
 Gene sequence
 Genotype
 Mycelium
 Nonhuman
 Phenotypic variation
 Soil
 Streptomyces
 Streptomyces actinomycinicus
 Streptomyces echinatus
 Streptomyces musisoli
 Bacterium identification
 Chemistry
 Classification
 DNA sequence
 Genetics
 Isolation and purification
 Microbiology
 Musa
 Phylogeny
 Rhizosphere
 Streptomyces
 Thailand
 Bacterial Typing Techniques
 Base Composition
 Diaminopimelic Acid
 DNA, Bacterial
 Fatty Acids
 Musa
 Mycelium
 Peptidoglycan
 Phospholipids
 Phylogeny
 Rhizosphere
 RNA, Ribosomal, 16S
 Sequence Analysis, DNA
 Soil Microbiology
 Streptomyces
 Thailand
 Vitamin K 2

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URI

https://hdl.handle.net/20.500.14740/8025

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Scopus: Year 1983-2021

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