Publication:
Selected activities of Citrus Maxima merr. Fruits on human endothelial cells: Enhancing cell migration and delaying cellular aging

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

20726643

DOI

10.3390/nu6041618

Other identifier(s)

2-s2.0-84899134634

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Nutrients. Vol 6, No.4 (2014), p.1618-1634

Suggested Citation

Buachan P., Chularojmontri L., Wattanapitayakul S.K. Selected activities of Citrus Maxima merr. Fruits on human endothelial cells: Enhancing cell migration and delaying cellular aging. Nutrients. Vol 6, No.4 (2014), p.1618-1634. doi:10.3390/nu6041618 Retrieved from: https://hdl.handle.net/20.500.14740/6782

Title

Selected activities of Citrus Maxima merr. Fruits on human endothelial cells: Enhancing cell migration and delaying cellular aging

Author(s)

Buachan P.
 Chularojmontri L.
 Wattanapitayakul S.K.

Abstract

Endothelial injury and damage as well as accumulated reactive oxygen species (ROS) in aging play a significant role in the development of cardiovascular disease (CVD). Recent studies show an association of high citrus fruit intake with a lower risk of CVD and stroke but the mechanisms involved are not fully understood. This study investigated the effects of pummelo (Citrus maxima Merr. var. Tubtim Siam, CM) fruit extract on human umbilical vein endothelial cell (HUVECs) migration and aging. The freeze-dried powder of fruit extract was characterized for antioxidant capacity (FRAP assay) and certain natural antioxidants, including ascorbic acid, gallic acid, hesperidin, and naringin (HPLC). Short-term (48 h) co-cultivation of HUVECs with CM enhanced cell migration as evaluated by a scratch wound assay and Boyden chamber assay. A long-term treatment with CM for 35 days significantly increased HUVEC proliferation capability as indicated by population doubling level (PDL). CM also delayed the onset of aging phenotype shown by senescence-associated β-galactosidase (SA-β-gal) staining. Furthermore, CM was able to attenuate increased ROS levels in aged cells when determined by 2′,7′-dichlorodihydrofluorescein diacetate (DCDHF) while eNOS mRNA expression was increased but the eNOS protein level was not changed. Thus, further in vivo and clinical studies are warranted to support the use of pummelo as a functional fruit for endothelial health and CVD risk reduction. © 2014 by the authors; licensee MDPI, Basel, Switzerland.

Subject(s)

Ascorbic acid
 Aurantiin
 Flavonoid
 Gallic acid
 Hesperidin
 Messenger RNA
 Plant extract
 2',7'-dichlorodihydrofluorescein diacetate
 Antioxidant
 Ascorbic acid
 Endothelial nitric oxide synthase
 Flavanone derivative
 Fluorescein derivative
 Gallic acid
 Hesperidin
 NOS3 protein, human
 Plant extract
 Reactive oxygen metabolite
 Antioxidant activity
 Article
 Cell aging
 Cell migration
 Centrifugation
 Citrus maxima
 Controlled study
 Endothelium cell
 Fluorescence recovery after photobleaching
 High performance liquid chromatography
 Human
 Human cell
 Oxidative stress
 Polyacrylamide gel electrophoresis
 Polymerase chain reaction
 Protein expression
 Pummelo
 Reverse transcription polymerase chain reaction
 Senescence
 Western blotting
 Wound healing
 Cell aging
 Cell motion
 Chemistry
 Citrus
 Drug effects
 Fruit
 Genetics
 Metabolism
 Umbilical vein endothelial cell
 Antioxidants
 Ascorbic Acid
 Cell Aging
 Cell Movement
 Citrus
 Flavanones
 Fluoresceins
 Fruit
 Gallic Acid
 Hesperidin
 Human Umbilical Vein Endothelial Cells
 Humans
 Nitric Oxide Synthase Type III
 Plant Extracts
 Reactive Oxygen Species
 RNA, Messenger

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URI

https://hdl.handle.net/20.500.14740/6782

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Scopus: Year 1983-2021

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