Publication:
Gold nanoparticle amplification combined with quartz crystal microbalance DNA based biosensor for detection of mycobacterium tuberculosis

2
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

17265479

Other identifier(s)

2-s2.0-84873978726

Rights Holder(s)

Scopus

Bibliographic Citation

Sensors and Transducers. Vol 146, No.11 (2012), p.156-163

Suggested Citation

Kaewphinit T., Santiwatanakul S., Chansiri K. Gold nanoparticle amplification combined with quartz crystal microbalance DNA based biosensor for detection of mycobacterium tuberculosis. Sensors and Transducers. Vol 146, No.11 (2012), p.156-163. Retrieved from: https://hdl.handle.net/20.500.14740/6879

Title

Gold nanoparticle amplification combined with quartz crystal microbalance DNA based biosensor for detection of mycobacterium tuberculosis

Author(s)

Kaewphinit T.
 Santiwatanakul S.
 Chansiri K.

Abstract

Tuberculosis is among the top ten causes of global mortality and morbidity, constitutes an important public health problem in Thailand. This study demonstrates a rapid and sensitive quartz crystal microbalance (QCM) biosensor combined with gold nanoparticle amplification for diagnosis Mycobacterium tuberculosis. The functionalized gold electrode of the quartz crystal was immobilized using the specific thiol modified oligonucleotide probe. The sensitivity was also improved by using gold nanoparticle as mass enhancement at 3' end of DNA target sequence. The target IS6110 amplified DNA was hybridized to a probe for 5 min after application. DNA hybridization was indicated by resulted in frequency changes of the QCM. The QCM could clearly identify the data indicated that QCM could detect serial dilution of M. tuberculosis DNA limited as 5 pg of genomic DNA. The technique showed a significant specificity since no cross-hybridization with other mycobacteria was found. In testing in direct clinical sputum samples, the sensitivity of QCM was 100 %, and the specificity was 100% by comparison with culture. The label free QCM was successfully developed with high sensitivity, specificity, low cost and convenience, this technique may prove to be a powerful tool for an early diagnosis of M. tuberculosis. © 2012 IFSA.

Subject(s)

Cross-hybridization
 DNA biosensors
 DNA hybridization
 DNA targets
 DNA-based biosensors
 Early diagnosis
 Frequency changes
 Functionalized
 Genomic DNA
 Gold electrodes
 Gold Nanoparticles
 High sensitivity
 Label free
 Low costs
 M. tuberculosis
 Mass enhancement
 Modified oligonucleotides
 Mycobacterium tuberculosis
 Quartz crystal
 Serial dilutions
 Sputum samples
 Thailand
 Tuberculosis
 Biosensors
 DNA
 Gold
 Metal nanoparticles
 Oligonucleotides
 Probes
 Quartz
 Tubes (components)
 Quartz crystal microbalances

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6879

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By