Publication:
Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice

0
0

Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

20366590

DOI

10.3855/jidc.14472

Other identifier(s)

2-s2.0-85118305440

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Infection in Developing Countries. Vol 15, No.9 (2021), p.328-1338

Suggested Citation

Aunguldee T., Gerdprasert O., Tangteerawatana P., Jariyapongskul A., Leelayoova S., Wongsatayanon B.T. Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice. Journal of Infection in Developing Countries. Vol 15, No.9 (2021), p.328-1338. doi:10.3855/jidc.14472 Retrieved from: https://hdl.handle.net/20.500.14740/14019

Title

Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice

Author(s)

Aunguldee T.
 Gerdprasert O.
 Tangteerawatana P.
 Jariyapongskul A.
 Leelayoova S.
 Wongsatayanon B.T.

Abstract

Introduction: In Thailand, Leishmania martiniquensis is the predominant species causing cutaneous and visceral leishmaniasis. Its incidence has been increasing among immunocompetent and immunocompromised hosts. We developed a prototype DNA vaccine using a partial consensus sequence of the cysteine protease B (cpb) gene derived from L. martiniquensis from Thai patients. Methodology: The laboratory inbred strain of albino BALB/c mice were immunized intramuscularly three times at 2-week intervals (weeks 0, 2, and 4) with cpb plasmid DNA (pcDNA_cpb) with or without the adjuvant, monoolein (pcDNA_cpb-MO). Mice were challenged at week 6 with L. martiniquensis promastigotes. Sera were analysed for IgG1, IgG2a, interferon gamma and interleukin 10 (IFN-γ and IL-10, respectively) levels at weeks 0, 4, and 9. Additionally, livers and spleens were also analysed for parasite burden using immunohistochemistry and real-time polymerase chain (qPCR) assays. Results: Three weeks after promastigote challenge, vaccinated mice showed significantly increased levels of IgG2a and IFN-γ while IL-10 level was significantly reduced when compared with those in the control group (p < 0.01). Parasite burden in the livers and spleens of vaccinated mice significantly decreased. In addition, a significant increase in mature granuloma formation in the livers when compared with those of the control group (p < 0.05) was found, indicating increased T-helper cells (Th1)-induced inflammation and destruction of amastigotes. Monoolein produced a booster effect to enhance the mouse Th1 protective immunity. Conclusions: The prototype DNA vaccine could induce a Th1 immune response that conferred potential protection to the L. martiniquensis promastigote challenge in BALB/c mice. © 2021 Aunguldee et al.

Subject(s)

Cell protein
 DNA vaccine
 Gamma interferon
 Glutamine
 Glycerol oleate
 Immunoglobulin G1
 Immunoglobulin G2a
 Interleukin 10
 Penicillin derivative
 Plasmid DNA
 Streptomycin
 DNA vaccine
 IL10 protein, human
 Interleukin 10
 Animal experiment
 Animal model
 Animal tissue
 Antibody response
 Article
 Controlled study
 Cpb gene
 Escherichia coli
 Experimental inflammation
 F Leishmania martiniquensis
 Gene
 Granuloma
 Immune response
 Immunization
 Immunofluorescence
 Immunogenicity
 Immunohistochemistry
 Inflammation
 Leishmania
 Leishmaniasis
 Mouse
 Nonhuman
 Parasite load
 Promastigote
 Protein expression
 Real time polymerase chain reaction
 Recombinant plasmid
 Th1 cell
 Animal
 Bagg albino mouse
 Blood
 Cutaneous leishmaniasis
 Female
 Human
 Immunology
 Leishmania
 Thailand
 Vaccination
 Visceral leishmaniasis
 Animals
 Female
 Humans
 Interleukin-10
 Leishmania
 Leishmaniasis, Cutaneous
 Leishmaniasis, Visceral
 Mice
 Mice, Inbred BALB C
 Thailand
 Vaccination
 Vaccines, DNA

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/14019

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By