Publication:
Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

11073756

DOI

10.3892/ijmm.2017.2939

Other identifier(s)

2-s2.0-85018742592

Rights Holder(s)

Scopus

Bibliographic Citation

International Journal of Molecular Medicine. Vol 39, No.5 (2017), p.1083-1090

Suggested Citation

Kuanpradit C., Jaisin Y., Jungudomjaroen S., Mitu S.A., Puttikamonkul S., Sobhon P., Cummins S.F. Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts. International Journal of Molecular Medicine. Vol 39, No.5 (2017), p.1083-1090. doi:10.3892/ijmm.2017.2939 Retrieved from: https://hdl.handle.net/20.500.14740/4178

Title

Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts

Author(s)

Kuanpradit C.
 Jaisin Y.
 Jungudomjaroen S.
 Mitu S.A.
 Puttikamonkul S.
 Sobhon P.
 Cummins S.F.

Abstract

Exposure to solar ultraviolet B (UV-B) is a known causative factor for many skin complications such as wrinkles, black spots, shedding and inflammation. Within the wavelengths 280-320 nm, UV-B can penetrate to the epidermal level. This investigation aimed to test whether extracts from the tropical abalone [Haliotis asinina (H. asinina)] mucus-secreting tissues, the hypobranchial gland (HBG) and gills, were able to attenuate the inflammatory process, using the human keratinocyte HaCaT cell line. Cytotoxicity of abalone tissue extracts was determined using an AlamarBlue viability assay. Results showed that HaCaT cells could survive when incubated in crude HBG and gill extracts at concentrations between <11.8 and <16.9 μg/ml, respectively. Subsequently, cell viability was compared between cultured HaCaT cells exposed to serial doses of UV-B from 1 to 11 (x10) mJ/cm2 and containing 4 different concentrations of abalone extract from both the HBG and gill (0, 0.1, 2.5, 5 μg/ml). A significant increase in cell viability was observed (P<0.001) following treatment with 2.5 and 5 μg/ml extract. Without extract, cell viability was significantly reduced upon exposure to UV-B at 4 mJ/cm2. Three morphological changes were observed in HaCaT cells following UV-B exposure, including i) condensation of cytoplasm; ii) shrunken cells and plasma membrane bubbling; and iii) condensation of chromatin material. A calcein AM-propidium iodide live-dead assay showed that cells could survive cytoplasmic condensation, yet cell death occurred when damage also included membrane bubbling and chromatin changes. Western blot analysis of HaCaT cell COX-2, p38, phosphor-p38, SPK/JNK and phosphor-SPK/JNK following exposure to >2.5 μg/ml extract showed a significant decrease in intensity for COX-2, phosphor-p38 and phosphor-SPK/JNK. The present study demonstrated that abalone extracts from the HGB and gill can attenuate inflammatory proteins triggered by UV-B. Hence, the contents of abalone extract, including cellmetabolites and peptides, may provide new agents for skin anti-inflammation, preventing damage due to UV-B.

Subject(s)

Cyclooxygenase 2
 Mitogen activated protein kinase p38
 Natural product
 Stress activated protein kinase
 Autacoid
 Biological marker
 Biological product
 Abalone
 Adult
 Animal tissue
 Antiinflammatory activity
 Article
 Cell membrane
 Cell structure
 Cell survival
 Chromatin condensation
 Controlled study
 Cytoplasm
 Cytotoxicity
 Female
 Gill
 Haliotis asinina
 Human
 Human cell
 Inflammation
 Keratinocyte
 Male
 Nonhuman
 Radiation exposure
 Ultraviolet B radiation
 Adverse effects
 Animal
 Cell culture
 Cell line
 Chemistry
 Drug effects
 Inflammation
 Keratinocyte
 Metabolism
 Secretion (process)
 Signal transduction
 Ultraviolet radiation
 Animals
 Biological Products
 Biomarkers
 Cell Line
 Cell Survival
 Cells, Cultured
 Female
 Gills
 Humans
 Inflammation
 Inflammation Mediators
 Keratinocytes
 Male
 Signal Transduction
 Ultraviolet Rays

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URI

https://hdl.handle.net/20.500.14740/4178

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Scopus: Year 1983-2021

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