Publication: In vitro Study of Antibiotic Susceptibility of Propionibacterium acnes Strains Isolated from Acne Vulgaris Patients
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Issued Date
2017
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85075027597
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S24-S31
Suggested Citation
Laochunsuwan A., Taweechotipatr M., Udompataikul M. In vitro Study of Antibiotic Susceptibility of Propionibacterium acnes Strains Isolated from Acne Vulgaris Patients. Journal of the Medical Association of Thailand. Vol 100, No.10 (2017), p.S24-S31. Retrieved from: https://hdl.handle.net/20.500.14740/4396
Author(s)
Abstract
Background: Antibiotic-resistant Propionibacterium acnes has become a worldwide problem in recent years. The prevalence of antibiotic resistance to this bacterium has increased in many countries. Over the past decade, there are few previous studies regarding the incidence of antibiotic resistance and antimicrobial susceptibility of P. acnes in Thailand. Objective: The aim of the present study is to determine the antibiotic susceptibility patterns of P. acnes strains from acne patients in Thailand. Material and Method: A cross sectional was used to investigate in this study. Ninety-five acne patients were enrolled. Samples were collected from facial closed comedones using comedone extraction technique and isolated in an anaerobic condition. The strains were identified by phenotypic characteristics and API 20A. Antibiotic susceptibility tests and minimal inhibitory concentration (MIC) of P. acnes strains to five antibiotics that are commonly used in acne treatments in Thailand (erythromycin, clindamycin, tetracycline, doxycycline and amoxicillin), were determined by the Epsilometer test method. Results: Among 95 samples, P. acnes strains were isolated from 75 patients (78.95%). MIC90 of doxycycline, tetracycline, amoxicillin, clindamycin and erythromycin were 1.7, 16, 0.016, 256 and 256 μg/mL, respectively. By using CLSI breakpoints for resistance, forty-eight (64%) and forty-seven (62.66%) strains resisted to erythromycin and clindamycin, while only one (1.33%) strain resisted to tetracycline. No resistance to doxycycline and amoxicillin was found in this present study. Moreover, there were statistically significant differences among age groups, history of previous antibiotic treatment and macrolide antibiotic resistance. Conclusion: Among the antibiotics tested in the present study, the most common antibiotic resistance was erythromycin, followed by clindamycin and tetracycline, respectively; whereas, no resistance to doxycycline and amoxicillin was found. The antibiotic-resistant P. acnes strains have been continuously increasing in Thai acne patients from the present study. © 2017 Medical Association of Thailand. All rights reserved.
Subject(s)
Amoxicillin
Arabinose
Beta glucosidase
Clindamycin
Doxycycline
Erythromycin
Glycerol
Isotretinoin
Lactose
Maltose
Mannitol
Mannose
Sucrose
Tetracycline
Urease
Acne vulgaris
Adult
Antibiotic sensitivity
Antibiotic therapy
Article
Bacterium isolation
CNKI database
Disease duration
Epsilometer test
Female
Human
Major clinical study
Male
Minimum inhibitory concentration
Phenotype
Propionibacterium acnes
Young adult
Arabinose
Beta glucosidase
Clindamycin
Doxycycline
Erythromycin
Glycerol
Isotretinoin
Lactose
Maltose
Mannitol
Mannose
Sucrose
Tetracycline
Urease
Acne vulgaris
Adult
Antibiotic sensitivity
Antibiotic therapy
Article
Bacterium isolation
CNKI database
Disease duration
Epsilometer test
Female
Human
Major clinical study
Male
Minimum inhibitory concentration
Phenotype
Propionibacterium acnes
Young adult